Determination of acriflavine, rivanol, Acridine Orange, Acridine Yellow and proflavine by a catalytic photokinetic method.

A fast and sensitive photokinetic method for the determination of acriflavine, rivanol, Acridine Orange, Acridine Yellow and proflavine is described. It is based on the rate of photoreduction of methylviologen in the presence of EDTA, sensitized by these diaminoacridines. The rate is monitored spectrophotometrically by the formation of ferroin, which is generated after reduction of iron(III) by the methylviologen cation radical in the presence of 1,10-phenanthroline. The method has been successfully applied to the determination of acriflavine and rivanol in pharmaceuticals.