Dunning J W, Stewart J T
Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Georgia, Athens, GA 30602, U.S.A.
Talanta. 1991 Jun;38(6):631-5. doi: 10.1016/0039-9140(91)80147-r.
Some new 9-substituted 10-methylacridinium trifluoromethanesuifonate (triflate) salts have been synthesized and shown to react in methanol with the model aromatic and aliphatic amines, aniline and n-butylamine, to form derivatives which absorb strongly at 445 and 439 nm, respectively. The color development is affected by heat and heating time and by the quantity of acridinium triflate used. A 10-50-fold molar excess of the triflate should be used and the solution heated at 60 degrees for 30 min. The linearity and reproducibility of the assay are improved by the presence of pyridine (for aniline) and triethylamine (for n-butylamine) in the reaction mixture. Beer's law is obeyed over the range 0-1860 ng/ml for aniline and 0-1440 ng/ml for n-butylamine, with each of the new reagents. The relative error and the precision of determination depends on the acridinium triflate used.
一些新的9-取代10-甲基吖啶三氟甲磺酸盐（三氟甲磺酸盐）已被合成，并显示在甲醇中与模型芳香胺和脂肪胺、苯胺和正丁胺反应，分别形成在445和439nm处有强烈吸收的衍生物。显色受加热温度、加热时间和所用三氟甲磺酸吖啶量的影响。应使用摩尔过量10 - 50倍的三氟甲磺酸盐，并将溶液在60℃加热30分钟。反应混合物中存在吡啶（用于苯胺）和三乙胺（用于正丁胺）可提高测定的线性和重现性。对于每种新试剂，苯胺在0 - 1860 ng/ml范围内、正丁胺在0 - 1440 ng/ml范围内符合比尔定律。测定的相对误差和精密度取决于所用的三氟甲磺酸吖啶。
