基于与1-氟-2,4-二硝基苯的胶束催化反应的流动注射停流动力学分光光度法测定药物。

Flow-injection stopped-flow kinetic spectrophotometric determination of drugs, based on micellar-catalysed reaction with 1-fluoro-2,4-dinitrobenzene.

作者信息

Georgiou C A, Koupparis M A, Hadjiioannou T P

机构信息

Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Panepistimiopolis, Kouponia, Athens 15771, Greece.

出版信息

Talanta. 1991 Jul;38(7):689-96. doi: 10.1016/0039-9140(91)80187-5.

DOI:10.1016/0039-9140(91)80187-5

PMID:18965207

Abstract

A flow-injection stopped-flow kinetic spectrophotometric method for the determination of hydrazines, hydrazides, amines and amino-acids, based on the cetyltrimethylammonium bromide catalysed reaction with 1-fluoro-2,4-dinitrobenzene is described. With the proposed method dihydralazine, isoniazid, levodopa and aspartame can be determined at concentrations of 0.1-6 x 10(-4)M. The calibration ranges can be varied by adjusting the pH and surfactant concentration. The determination of amphetamine, cysteine, s-carboxymethylcysteine, cephalexin, tobramycin and gentamicin is also feasible. The method has been applied to the determination of levodopa, isoniazid and aspartame in commercial pharmaceutical formulations. The determination of isoniazid in formulations containing the highly coloured antibiotic rifamycin, and of aspartame in coloured beverages was also accomplished. The results were in good agreement with those obtained by reference methods and the throughput was 40 measurements per hour with 0.4-3.9% RSD.

摘要

描述了一种流动注射停流动力学分光光度法，用于测定肼类、酰肼类、胺类和氨基酸类，该方法基于十六烷基三甲基溴化铵催化的与1-氟-2,4-二硝基苯的反应。采用该方法，可在0.1 - 6×10⁻⁴M的浓度范围内测定双肼屈嗪、异烟肼、左旋多巴和阿斯巴甜。校准范围可通过调节pH值和表面活性剂浓度来改变。测定苯丙胺、半胱氨酸、S-羧甲基半胱氨酸、头孢氨苄、妥布霉素和庆大霉素也是可行的。该方法已应用于市售药物制剂中左旋多巴、异烟肼和阿斯巴甜的测定。还完成了含高度显色抗生素利福霉素制剂中异烟肼的测定以及有色饮料中阿斯巴甜的测定。结果与参考方法所得结果吻合良好，每小时可进行40次测量，相对标准偏差为0.4 - 3.9%。