邻苯二甲醛-硫酸作为一种喷雾试剂在某些吲哚烷基胺的薄层色谱检测中的应用及对蟾蜍皮肤分泌提取物的应用

Utilization of O-phthalaldehyde-sulphuric acid as a spray reagent in thin-layer chromatographic detection of some indolealkylamines and application to cutaneous secretion extracts of toad species.

作者信息

De Lima C G, Pastore T C, Schwartz C A, Cruz J S, Sebben A

机构信息

Departamento de Química, Universidade de Brasília, Brasília, DF 70910, Brazil.

出版信息

Talanta. 1991 Nov;38(11):1303-7. doi: 10.1016/0039-9140(91)80110-l.

DOI:10.1016/0039-9140(91)80110-l

PMID:18965302

Abstract

An expansion of the utilisation of o-phthalaldehyde in sulphuric acid medium as spray reagent was carried out when tryptophan and some tryptophan-derived indole alkylamines such as tryptamine, serotonin, bufotenine, dehydrobufotenine and bufotenidine were examined by thin-layer chromatography. Rf-values and limits of detection ranging from 20 (serotonin) to 100 (dehydrobufotenine) ng per spot were found. Application of this reagent for the detection of some of these compounds was carried out, using either methanolic extracts or column chromatographic fractions of the skin secretion of the toads Bufo ictericus and Odontophrynus cultripes.

摘要

当通过薄层色谱法检测色氨酸和一些色氨酸衍生的吲哚烷基胺（如色胺、血清素、蟾毒色胺、脱氢蟾毒色胺和蟾毒配基）时，对邻苯二甲醛在硫酸介质中作为喷雾试剂的应用进行了扩展。发现比移值（Rf值）和检测限为每点20（血清素）至100（脱氢蟾毒色胺）纳克。使用蟾蜍Bufo ictericus和Odontophrynus cultripes皮肤分泌物的甲醇提取物或柱色谱馏分，应用该试剂检测其中一些化合物。