共荧光效应在时间分辨荧光法检测铕、铽、钐和镝中的分析应用。

Analytical application of the co-fluorescence effect in detection of europium, terbium, samarium and dysprosium with time-resolved fluorimetry.

作者信息

Xu Y Y, Hemmilä I A

机构信息

Institute of Atomic Energy of China, Analytical Chemistry Laboratory, P.O. Box 275-88, Beijing, China; Wallac Biochemical Laboratory, P.O. Box 10, 20101, Turku, Finland.

出版信息

Talanta. 1992 Jul;39(7):759-63. doi: 10.1016/0039-9140(92)80091-q.

DOI:10.1016/0039-9140(92)80091-q

PMID:18965447

Abstract

Application of the co-fluorescence effect has been examined for the simultaneous detection of the lanthanide ions Eu(3+), Tb(3+), Sm(3+) and Dy(3+). In the presence of Y(3+) and 2,2'-bipyridine (BP), the fluorescence intensities of the pivaloyltrifluoroacetone chelates of these lanthanides were greatly enhanced by an inter-chelate energy transfer process. Under optimized conditions, the following detection limits were obtained; 0.019 pM for Eu(3+), 0.27 pM for Tb(3+), 3.8 pM for Sm(3+) and 20 pM for Dy(3+), when a sensitive time-resolved fluorometer was used for the measurement. A co-fluorescence based fluorescence enhancement solution was also tested in a dual-label time-resolved immunofluorometric assay of luteinizing and follicle stimulating hormones (LH and FSH) based on the use of Eu(3+) and Tb(3+) as the label ions. The present fluorometric detection system is particularly well suited for multilabel time-resolved fluorometric immunoassays utilizing two, three or four ion labels simultaneously.

摘要

已对共荧光效应在同时检测镧系离子铕（Eu(3+)）、铽（Tb(3+)）、钐（Sm(3+)）和镝（Dy(3+)）中的应用进行了研究。在钇（Y(3+)）和2,2'-联吡啶（BP）存在的情况下，这些镧系元素的新戊酰三氟丙酮螯合物的荧光强度通过螯合物间能量转移过程得到了极大增强。在优化条件下，当使用灵敏的时间分辨荧光计进行测量时，获得了以下检测限：铕（Eu(3+)）为0.019 pM，铽（Tb(3+)）为0.27 pM，钐（Sm(3+)）为3.8 pM，镝（Dy(3+)）为20 pM。一种基于共荧光的荧光增强溶液也在基于使用铕（Eu(3+)）和铽（Tb(3+)）作为标记离子的促黄体生成素和促卵泡生成素（LH和FSH）的双标记时间分辨免疫荧光测定中进行了测试。本荧光检测系统特别适用于同时使用两个、三个或四个离子标记的多标记时间分辨荧光免疫测定。