在硫酸介质中，以三（1,10 - 菲啰啉）铁（III）配合物作为氧化剂，采用流动注射比色法测定药物制剂中的维生素C。

Flow injection colorimetric method for the assay of vitamin C in drug formulations using tris,1-10-phenanthroline-iron(III) complex as an oxidant in sulfuric acid media.

作者信息

Sultan S M, Abdennabi A M, Suliman F E

机构信息

Chemistry Department, King Fahd University of Petroleum and Minerals, Dhahran, Saudi Arabia.

出版信息

Talanta. 1994 Jan;41(1):125-30. doi: 10.1016/0039-9140(94)80177-0.

DOI:10.1016/0039-9140(94)80177-0

PMID:18965896

Abstract

A simple, fast and accurate colorimetric flow injection (FI) method suitable for the assay of vitamin C in drug formulations was proposed. In the method, vitamin C was injected into a flowing stream of iron(III) and then mixed with 1,10-phenanthroline in 0.05M sulphuric acid media. The mixture was allowed to react in a 45-cm long coil and the resulting solution of tris, 1-10-phenanthroline-iron(II) complex was monitored at 510 nm. The method was adopted by fully investigating the kinetics of the reaction and proposing a suitable mechanism. A throughput of 100 samples per hour was achieved with a relative standard deviation of 0.88% for vitamin C concentration range of 100-400 ppm.

摘要

提出了一种简单、快速且准确的比色流动注射（FI）法，适用于药物制剂中维生素C的测定。在该方法中，将维生素C注入铁（III）的流动流中，然后在0.05M硫酸介质中与1,10 - 菲啰啉混合。使混合物在45厘米长的盘管中反应，并在510纳米处监测生成的三（1,10 - 菲啰啉）铁（II）配合物溶液。通过充分研究反应动力学并提出合适的机理来采用该方法。对于100 - 400 ppm的维生素C浓度范围，每小时可实现100个样品的通量，相对标准偏差为0.88%。