导数可变偏移同步荧光光谱法快速同时分析水样中的1-萘酚和2-萘酚

Rapid simultaneous analysis of 1-naphthol and 2-naphthol in water samples by derivative variable-offset synchronous fluorescence spectroscopy.

作者信息

Yao Q L, Xian Z H, Jin G X, Guo Z C

机构信息

Department of Chemistry, Xiamen University, Xiamen 361005, People's Republic of China.

出版信息

Talanta. 1994 May;41(5):695-701. doi: 10.1016/0039-9140(94)80051-0.

DOI:10.1016/0039-9140(94)80051-0

PMID:18965985

Abstract

Derivative variable-offset synchronous fluorescence spectroscopy is developed to improve the spectral resolution and the selectivity of fluorescence measurements. 1-naphthol and 2-naphthol are employed to evaluate the proposed coupled technique and the various spectral comparisons are conducted. Second derivative variable-offset synchronous scanning permits the rapid simultaneous identification and quantitative determination of 1-naphthol and 2-naphthol in a mixture from a single spectrum. 6.7-2000 ng/ml 1-naphthol and 3.6-500 ng/ml 2-naphthol can be quantified with 1-naphthol and 2-naphthol ratios of 40: 1-1: 10. The determination of 1-naphthol and 2-naphthol in various spiked water samples gave a mean recovery of 100.7% with a relative standard deviation of 2.8% for 1-naphthol and mean recovery of 99.7% with a relative standard deviation (RSD of 2.6% for 2-naphthol, respectively.

摘要

为提高荧光测量的光谱分辨率和选择性，开发了导数可变偏移同步荧光光谱法。采用1-萘酚和2-萘酚对所提出的联用技术进行评估，并进行了各种光谱比较。二阶导数可变偏移同步扫描可从单一光谱中快速同时鉴定和定量混合物中的1-萘酚和2-萘酚。1-萘酚和2-萘酚的比例为40:1至1:10时，6.7-2000 ng/ml的1-萘酚和3.6-500 ng/ml的2-萘酚可被定量。对各种加标水样中1-萘酚和2-萘酚的测定结果表明，1-萘酚的平均回收率为100.7%，相对标准偏差为2.8%；2-萘酚的平均回收率为99.7%，相对标准偏差为2.6%。