过氧化物酶催化鲁米诺化学发光法测定谷胱甘肽
Peroxidase-catalysed luminol chemiluminescence method for the determination of glutathione.
作者信息
Kamidate T, Watanabe H
机构信息
Faculty of Engineering, Hokkaido University, Kita-13, Nishi-8, Kita-ku, Sapporo, Japan.
出版信息
Talanta. 1996 Oct;43(10):1733-8. doi: 10.1016/0039-9140(96)01956-X.
A luminol chemiluminescence (CL) method was developed for the determination of glutathione (GSH). GSH was indirectly determined by measuring the amount of hydrogen peroxide formed during the Cu(II)-catalysed oxidation of GSH with oxygen. The amount of hydrogen peroxide formed was continuously measured using the Arthromyces ramosus peroxidase-catalysed luminol CL reaction. The CL intensities at maximum light emission were linearly correlated with the concentration of GSH over the range 7.5 x 10(-7)-3.0 x 10(-5) M. The detection limit for GSH was about 10 times better than that of the spectrophotometric method using Ellman reagent.
开发了一种鲁米诺化学发光（CL）法用于测定谷胱甘肽（GSH）。通过测量在Cu（II）催化的GSH与氧气氧化过程中形成的过氧化氢量来间接测定GSH。使用分枝杆菌过氧化物酶催化的鲁米诺CL反应连续测量形成的过氧化氢量。最大发光时的CL强度在7.5×10（-7）-3.0×10（-5）M范围内与GSH浓度呈线性相关。GSH的检测限比使用埃尔曼试剂的分光光度法约好10倍。
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