氨基硫醇在鲁米诺化学发光与铜（II）催化的半胱氨酸和谷胱甘肽氧化反应中的作用

Role of amino thiols in luminol chemiluminescence coupled with copper(II)-catalysed oxidation of cysteine and glutathione.

作者信息

Kamidate T, Kinkou T, Watanabe H

机构信息

Faculty of Engineering, Hokkaido University, Sapporo, Japan.

出版信息

J Biolumin Chemilumin. 1996 May-Jun;11(3):123-9. doi: 10.1002/(SICI)1099-1271(199605)11:3<123::AID-BIO405>3.0.CO;2-Z.

DOI:10.1002/(SICI)1099-1271(199605)11:3<123::AID-BIO405>3.0.CO;2-Z

PMID:8844342

Abstract

The Cu(II)-catalysed oxidation of the amino thiols cysteine and glutathione with oxygen was examined in the presence of luminol. Light emission was markedly suppressed when the concentration of amino thiols was greater than that of Cu(II). Cysteine caused a delay of chemiluminescence (CL) and no CL emission was observed at high concentrations of GSH. The suppression in CL could be explained in terms of the complexation of the Cu(II) catalyst by formation of a Cu(II)-dicysteine complex in case of cysteine, and of a Cu(II)-GSSG complex in case of GSH.

摘要

在鲁米诺存在的情况下，研究了铜（II）催化的氨基酸硫醇半胱氨酸和谷胱甘肽与氧气的氧化反应。当氨基酸硫醇的浓度高于铜（II）的浓度时，发光明显受到抑制。半胱氨酸导致化学发光（CL）延迟，在高浓度谷胱甘肽时未观察到CL发射。CL的抑制可以用以下方式解释：在半胱氨酸的情况下，通过形成铜（II）-二半胱氨酸络合物使铜（II）催化剂络合；在谷胱甘肽的情况下，通过形成铜（II）-氧化型谷胱甘肽络合物使铜（II）催化剂络合。