Inoue A, Earley R L, Lehmann M W, Welch L E
Department of Chemistry, Knox College, Galesburg, IL 61401 USA.
Talanta. 1998 Aug;46(6):1507-14. doi: 10.1016/s0039-9140(98)00018-6.
An examination was made of the effect of electrode polishing on the direct pulsed amperometric detection (PAD) and indirect PAD response to penicillin G. The polishing procedure produced a temporary loss of sensitivity for both types of PAD, which over time relaxed back to higher levels. A temporary increase in the electrode kinetics can be observed as well, particularly for a PAD waveform in the crossover region between direct and indirect PAD. In extreme cases, the penicillin G response can undergo a complete polarity reversal. By observing post-polish equilibration times, it was recommended that the direct and indirect PAD user observe 3 and 5 h delay times, respectively, to insure stable electrode response following an electrode polish.
