基于 1,10-菲啰啉存在下半胱氨酸与铁(III)的氧化还原反应中铜(II)的催化作用，用流动注射法测定铜(II)。

Flow-injection determination of copper(II) based on its catalysis on the redox reaction of cysteine with iron(III) in the presence of 1,10-phenanthroline.

机构信息

Laboratory of Analytical Chemistry, Department of Chemistry, University of Tsukuba, Tsukuba 305-8571, Japan.

出版信息

Talanta. 1999 Aug 23;50(1):41-7. doi: 10.1016/s0039-9140(99)00108-3.

Abstract

A redox reaction of cysteine with iron(III) proceeds slowly in the presence of 1,10-phenanthroline (phen). However, this reaction is accelerated in the presence of copper(II) as a catalyst, producing an iron(II)-phen complex (lambda(max)=510 nm). A sensitive spectrophotometric flow-injection method is proposed for the determination of copper(II) based on its catalytic action on this redox reaction. The dynamic range was 0.1-10 ng ml(-1) of copper(II) with a relative standard deviation of 1.0% (n=10) for 1.0 ng ml(-1) of copper(II) at a sampling rate of 30 h(-1). The detection limit (S/N=3) is 0.04 ng ml(-1). The proposed method was successfully applied to the determination of copper in river water as a certified reference material.

摘要

半胱氨酸与三价铁的氧化还原反应在 1,10-菲啰啉（phen）存在下进行得很慢。然而，在铜（II）作为催化剂存在下，该反应会加速，生成铁（II）-phen 配合物（lambda(max)=510nm）。本文提出了一种基于该氧化还原反应的铜（II）的灵敏分光光度流动注射法测定方法。铜（II）的动态范围为 0.1-10ngml(-1)，相对标准偏差为 1.0%（n=10），对于 1.0ngml(-1)的铜（II），采样率为 30h(-1)。检测限（S/N=3）为 0.04ngml(-1)。该方法成功地应用于河水铜的测定，作为一种有证参考物质。