Prioli R P, Mejia J S, Aji T, Aikawa M, Pereira M E
New England Medical Center Hospital, Department of Geographic Medicine and Infectious Diseases, Boston.
Trop Med Parasitol. 1991 Jun;42(2):146-50.
Immunoelectron microscopy (IEM) using TCN-2, a monoclonal antibody specific for Trypanosoma cruzi neuraminidase (NA), was performed to determine the precise localization of the parasite enzyme. In agreement with previous observations, TCN-2 reacted with tissue culture trypomastigotes, but not with epimastigotes, amastigotes or intracellular forms in intermediate stages of development. NA was localized on the surface of tissue culture trypomastigotes and in the Golgi apparatus suggesting that the enzyme is modified post-translationally. In agreement with this suggestion, digestion of NA with N-Glycanase, an enzyme that releases N-linked oligosaccharides, decreased the molecular weight of the polypeptides that make up NA.
利用针对克氏锥虫神经氨酸酶（NA）的单克隆抗体TCN - 2进行免疫电子显微镜（IEM）检测，以确定该寄生虫酶的精确定位。与先前的观察结果一致，TCN - 2与组织培养型锥鞭毛体发生反应，但不与上鞭毛体、无鞭毛体或发育中间阶段的细胞内形式发生反应。NA定位于组织培养型锥鞭毛体的表面和高尔基体中，这表明该酶在翻译后被修饰。与这一推测一致的是，用释放N - 连接寡糖的N - 聚糖酶消化NA后，构成NA的多肽分子量降低。
