Basavaiah K, Swamy J M
Department of Studies in Chemistry, University of Mysore, Manasagangotri, Mysore, India.
Talanta. 1999 Nov 15;50(4):887-92. doi: 10.1016/s0039-9140(99)00179-4.
A simple, rapid and sensitive spectrophotometric method has been developed for the assay of ceterizine hydrochloride (CTZH) in bulk drug and its pharmaceutical preparations. This method is based on the ion-pair complex reaction between CTZH and Alizarin Red S in Clarks-Lubs buffer. The chromogen being extractable with chloroform, could be measured quantitatively at 440 nm. All variables were studied to optimise the reaction conditions. Regression analysis of Beer's Law plot showed good correlation in the concentration range 2.5-22 microg ml(-1). The method has a detection limit of 0.1328 microg ml(-1). The proposed method has been successfully applied for the analysis of the bulk drug and its dosage forms such as tablets and syrups. No interference was observed from common pharmaceutical adjuvants.
已开发出一种简单、快速且灵敏的分光光度法，用于测定原料药及其药物制剂中的盐酸西替利嗪（CTZH）。该方法基于CTZH与茜素红S在克拉克-卢布斯缓冲液中发生的离子对络合反应。生成的色原可用氯仿萃取，并在440nm处进行定量测定。研究了所有变量以优化反应条件。比尔定律曲线的回归分析表明，在2.5-22μg ml(-1)的浓度范围内具有良好的相关性。该方法的检测限为0.1328μg ml(-1)。所提出的方法已成功应用于原料药及其剂型（如片剂和糖浆剂）的分析。未观察到常见药物辅料的干扰。
