Wang R Y, Jarratt J A, Keay P J, Hawkes J J, Coakley W T
Centre for Advanced Micro-Analytical Systems, Faculty of Science, Technology and Design, University of Luton, Park Square, Luton, Bedfordshire LU1 3JU, UK.
Talanta. 2000 May 31;52(1):129-39. doi: 10.1016/s0039-9140(99)00342-2.
The design and construction of an automated on-line analysis system is described with reference to applications in bioprocess control, clinical, and environmental analysis. The new system is built around three main elements: ultrasound filtration, a micro flow injection analysis (FIA) system, and direct readout spectrophotometry. The advantages of three on-line ultrasound filtration devices, developed for clarification of water, human blood and mammalian cell culture samples, are described. The filters avoid off-line centrifugation and do not suffer from the blockage problems associated with conventional filters. The separation efficiency of the ultrasound filters is also discussed. The delivery system is based on a gas driven FIA technique, using helium to avoid bubble formation, with the carrier and reagent flow being controlled by computer switched solenoid valves. Direct readout spectrometers are used, based on charge coupled devices (CCDs) covering the wavelength range 200-900 nm. These detectors provide near instantaneous capture of full spectra, allowing several analytes to be monitored simultaneously, and are much smaller than conventional spectrophotometers. Optical fibres are used to link the light source to the detector via a flow cell in the FIA system. Software to run the entire system was developed using the LabVIEWtrade mark graphical programming environment, enabling rapid development of the control system and user interface. The integration of these components has shown significant improvement in the application of FIA techniques to on-line analysis.
本文介绍了一种自动化在线分析系统的设计与构建，并参考了其在生物过程控制、临床和环境分析中的应用。新系统围绕三个主要元素构建：超声过滤、微流动注射分析（FIA）系统和直读分光光度法。文中描述了为净化水、人体血液和哺乳动物细胞培养样品而开发的三种在线超声过滤装置的优点。这些过滤器避免了离线离心，并且不存在与传统过滤器相关的堵塞问题。还讨论了超声过滤器的分离效率。输送系统基于气体驱动的FIA技术，使用氦气以避免气泡形成，载体和试剂流由计算机控制的电磁阀控制。使用覆盖200 - 900 nm波长范围的电荷耦合器件（CCD）的直读光谱仪。这些探测器能够近乎即时地捕获全光谱，允许同时监测多种分析物，并且比传统分光光度计小得多。光纤用于通过FIA系统中的流通池将光源与探测器连接起来。使用LabVIEW商标图形编程环境开发了运行整个系统的软件，从而能够快速开发控制系统和用户界面。这些组件的集成在将FIA技术应用于在线分析方面显示出显著的改进。
