Warmke H, Wiczk W, Ossowski T
Faculty of Chemistry, University of Gdańsk, Sobieskiego 18, 80-952 Gdansk, Poland.
Talanta. 2000 Jun 30;52(3):449-56. doi: 10.1016/s0039-9140(00)00365-9.
The influence of metal cations Li(+), Na(+), K(+), Cs(+), Mg(2+), Ca(2+), Sr(2+), Ba(2+), Co(2+), Ni(2+), Cu(2+), Zn(2+), Pb(2+) and Al(3+) on the spectroscopic properties of the dansyl (1-dimethylaminonaphthalene-5-sulfonyl) group covalently linked to monoaza crown ethers 1-aza-15-crown-5 (1,4,7,10,-tetraoxa-13-azacyclopentadecane) (A15C5) and 1-aza-crown-6 (1,4,7,10,13-pentaoxa-16-azacyclooctadecane) (A18C6) was investigated by means of absorption and emission spectrophotometry. Interaction of the alkali metal ions with both fluoroionophores is weak, while alkaline earth metal ions interact strongly causing 50 and 85% quenching of dansyl fluorescence of N-(5-dimethylamine-1-naphthalenesulfonylo)-1,4,7,10,-tetraoxa-13-azacyclopentadecane (A15C5-Dns) and N-(5-dimethylamine-1-naphthalenesulfonylo)-1,4,7,10,13-pentaoxa-16-azacyclooctadecane (A18C6-Dns), respectively. The Cu(2+), Pb(2+) and Al(3+) cations interact very strongly with dansyl chromophore, causing a major change in absorption spectrum of the chromophore and forming non-fluorescent complexes. The Co(2+), Ni(2+), Zn(2+), Mg(2+) cations interact moderately with both fluoroionophores causing quenching of dansyl fluorescence by several percent only.
通过吸收和发射分光光度法研究了金属阳离子Li(+)、Na(+)、K(+)、Cs(+)、Mg(2+)、Ca(2+)、Sr(2+)、Ba(2+)、Co(2+)、Ni(2+)、Cu(2+)、Zn(2+)、Pb(2+)和Al(3+)对与单氮杂冠醚1-氮杂-15-冠-5（1,4,7,10-四氧杂-13-氮杂环十五烷）（A15C5）和1-氮杂-冠-6（1,4,7,10,13-五氧杂-16-氮杂环十八烷）（A18C6）共价连接的丹磺酰基（1-二甲基氨基萘-5-磺酰基）光谱性质的影响。碱金属离子与两种氟离子载体的相互作用较弱，而碱土金属离子相互作用强烈，导致N-(5-二甲胺基-1-萘磺酰基)-1,4,7,10-四氧杂-13-氮杂环十五烷（A15C5-Dns）和N-(5-二甲胺基-1-萘磺酰基)-1,4,7,10,13-五氧杂-16-氮杂环十八烷（A18C6-Dns）的丹磺酰荧光分别猝灭50%和85%。Cu(2+)、Pb(2+)和Al(3+)阳离子与丹磺酰发色团相互作用非常强烈，导致发色团吸收光谱发生重大变化并形成非荧光络合物。Co(2+)、Ni(2+)、Zn(2+)、Mg(2+)阳离子与两种氟离子载体适度相互作用，仅使丹磺酰荧光猝灭百分之几。
