Freire R S, Durán N, Kubota L T
Instituto de Química, Universidade Estadual de Campinas, CP 6154, CEP 13083-970, Campinas, SP, Brazil.
Talanta. 2001 May 30;54(4):681-6. doi: 10.1016/s0039-9140(01)00318-6.
Fungal laccase was immobilized on carbon-fiber electrodes using classical methods: physical adsorption, glutaraldehyde, carbodiimide and carbodiimide/glutaraldehyde. The highest biosensor response was obtained using carbodiimide/glutaraldehyde for coupling laccase to carboxyl groups on the carbon fibers. In this method, different percentages of glutaraldehyde had important effects on the sensitivity of the biosensor, the best percentage of glutaraldehyde being 10% (m/v). The behavior of the obtained biosensor was investigated in terms of sensitivity, operational range, pH and applied potential. The developed biosensor showed an optimum response at pH 5.0 and at an applied potential of -100 mV. The immobilized laccase retained a good activity for over 2 months.
物理吸附法、戊二醛法、碳二亚胺法以及碳二亚胺/戊二醛法。使用碳二亚胺/戊二醛将漆酶与碳纤维上的羧基偶联时，生物传感器获得了最高响应。在该方法中，不同百分比的戊二醛对生物传感器的灵敏度有重要影响，戊二醛的最佳百分比为10%（质量/体积）。从灵敏度、工作范围、pH值和施加电位等方面对所得生物传感器的性能进行了研究。所开发的生物传感器在pH 5.0和施加电位为 -100 mV时表现出最佳响应。固定化漆酶在两个多月的时间里都保持了良好的活性。
