药物制剂中氯巴占、氯氮䓬、氟西泮和氟硝西泮的测定。
Determination of Clobazam, Clorazepate, Flurazepam and Flunitrazepam in pharmaceutical preparations.
作者信息
Berzas J J, Castañeda G, Pinilla M J
机构信息
Departamento de Quimica Analitica y Tecnologia de Alimentos, Facultad de Quimicas, Universidad de Castilla-La Mancha, 13071 Ciudad Real, Spain.
出版信息
Talanta. 2002 May 16;57(2):333-41. doi: 10.1016/s0039-9140(02)00024-3.
The separation of four benzodiazepines (Flurazepam, Flunitrazepam, Clobazam and Clorazepate) in pharmaceutical products by micellar electrokinetic chromatography (MEKC) is described. It was carried out at 25 degrees C and 25 kV by using a 57 cm (50 cm to the detector)x75 mum i.d. fused silica capillary and a 15 mM borate buffer (pH 9.2), 35 mM sodium dodecylsulfate (SDS) and 35 mM sodium deoxycholate water solution. Under these conditions, the analysis was performed in 8 min with acceptable limits of quantification (between 3 and 5 mg l(-1)). The method has been applied for quantifying these benzodiazepines in serum and different commercial formulations with recoveries near 100%.
描述了采用胶束电动色谱法（MEKC）分离药品中的四种苯二氮䓬类药物（氟西泮、氟硝西泮、氯巴占和氯氮䓬）。该实验在25℃和25 kV条件下进行，使用一根57 cm（至检测器的长度为50 cm）×75 μm内径的熔融石英毛细管以及15 mM硼酸盐缓冲液（pH 9.2）、35 mM十二烷基硫酸钠（SDS）和35 mM脱氧胆酸钠水溶液。在这些条件下，8分钟内即可完成分析，定量限在可接受范围内（3至5 mg l⁻¹）。该方法已用于血清和不同市售制剂中这些苯二氮䓬类药物的定量分析，回收率接近100%。
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