Department of Analytical Chemistry and Foods Technology, University of Castilla-La Mancha, 13071, Ciudad Real, Spain.
Talanta. 2002 Jun 10;57(4):795-805. doi: 10.1016/s0039-9140(02)00105-4.
In the present paper, the modified simplex method (MSM) has been applied, for the first time, to determine compounds by a luminescence technique. The method was based on the optimization of chemical and instrumental variables affecting phosphorescence using a geometric simplex in two and three dimensions of space, respectively. As application, we have determined a novel antihypertensive drug, naftopidil, in urine and serum, by heavy atom induced room temperature phosphorescence (HAI-RTP); this technique enables us to determine analytes in complex matrices, biological fluids, without the need for a tedious prior separation process. With the proposed method, the maximum signal of phosphorescence appears instantly once the sample has been prepared and the intensity was measured at lambda(ex)=287 nm and lambda(em)=525 nm. Overall least-squares regression was used to find the straight line that fitted the experimental data. The detection limit, as well as the repeatability and the standard deviation (S.D.) for replicate sample, were also determined.
在本文中，我们首次应用改进单纯形法（MSM）通过荧光技术来测定化合物。该方法基于使用二维和三维空间中的几何单纯形分别优化影响磷光的化学变量和仪器变量。作为应用实例，我们通过重原子室温磷光（HAI-RTP）法在尿液和血清中测定了一种新型抗高血压药物萘哌地尔；该技术使我们能够在无需繁琐的预先分离过程的情况下，在复杂基质、生物流体中测定分析物。在提出的方法中，一旦制备好样品并在 lambda(ex)=287nm 和 lambda(em)=525nm 处测量强度，磷光的最大信号就会立即出现。我们使用总体最小二乘法来找到拟合实验数据的直线。还确定了检测限以及重复性和重复样品的标准偏差（S.D.）。
