Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Institute for Science and Technology Research and Development, Chiang Mai University, Chiang Mai 50200, Thailand.
Talanta. 2002 Dec 6;58(6):1319-26. doi: 10.1016/s0039-9140(02)00441-1.
Kinetic information for the phosphate-molybdate-ascorbic acid reaction can be obtained by making use of a very simple manually operated stopped-flow injection (FI) system. Various parameters (concentrations of reagents, flow rate, mixing coils, and volume of flow cell) were investigated for determination of phosphate. A stopped-FI system should be arranged for low degree of mixing (of reactants) and low dispersion so that good signals of rate changes will be observed. Simultaneous determination of phosphate and silicate by the stopped-FI technique is proposed, using a laboratory-made semi-automatic stopped-FI Analyzer with LED-based photometer. It is based on kinetic separation of phosphate and silicate using molybdenum blue. The proposed procedure has been demonstrated for the application to water samples. The results obtained agree with that of a standard method.
通过利用非常简单的手动停流注射（FI）系统，可以获得磷酸盐-钼酸盐-抗坏血酸反应的动力学信息。针对磷酸盐的测定，对各种参数（试剂浓度、流速、混合线圈和流通池体积）进行了研究。停流 FI 系统应设计为低混合度（反应物）和低分散度，以便观察到良好的速率变化信号。本文提出了一种使用基于 LED 的光度计的实验室自制半自动停流 FI 分析仪，通过停流 FI 技术同时测定磷酸盐和硅酸盐。该方法基于钼蓝动力学分离磷酸盐和硅酸盐。该方法已应用于水样的分析。所得结果与标准方法一致。
