胶束增敏 H 点标准加入法间接同时测定氨基脲和肼。

Indirect simultaneous kinetic determination of semicarbazide and hydrazine in micellar media by H-point standard addition method.

机构信息

Department of Chemistry, College of Sciences, Shiraz University, Shiraz 71454, Iran.

出版信息

Talanta. 2003 Jan 2;59(1):147-53. doi: 10.1016/s0039-9140(02)00465-4.

Abstract

H-point standard addition method (HPSAM) is suggested as a simple and selective method for the determination of semicarbazide and hydrazine. The reduction of Cu(2+) to Cu(+) by semicarbazide and hydrazine in the presence of neocuproine (Nc) and the subsequent complex formation between Cu(+) and Nc produced a sensitive spectrophotometric method for indirect determination of semicarbazide and hydrazine. The difference in the rate of reduction of Cu(2+) with semicarbazide and hydrazine in cationic micellar media is the basis of this method. Semicarbazide can be determined in the range of 0.5-3.75 mug ml(-1) with satisfactory accuracy and precision in the presence of excess hydrazine. The proposed method was successfully applied to the simultaneous determination of semicarbazide (0.5-3.75 mug ml(-1)) and hydrazine (0.5-5 mug ml(-1)) and also to the selective determination of semicarbazide in the presence of hydrazine in several synthetic mixtures containing different concentration ratios of semicarbazide and hydrazine.

摘要

H 点标准加入法（HPSAM）被建议作为一种简单而选择性的方法，用于测定氨基脲和肼。在新铜试剂（Nc）存在下，氨基脲和肼将 Cu(2+)还原为 Cu(+)，随后 Cu(+)与 Nc 形成配合物，这为间接测定氨基脲和肼提供了一种灵敏的分光光度法。该方法的基础是阳离子胶束介质中 Cu(2+)与氨基脲和肼还原速率的差异。在过量肼存在下，氨基脲可在 0.5-3.75 μg ml(-1)范围内进行测定，具有令人满意的准确度和精密度。该方法成功应用于同时测定氨基脲（0.5-3.75 μg ml(-1))和肼（0.5-5 μg ml(-1))，以及在含有不同浓度比的氨基脲和肼的几种合成混合物中选择性测定氨基脲。