固相萃取高效液相色谱-光诱导荧光检测法监测河水中的苯脲和丙草胺除草剂。

Monitoring of phenylurea and propanil herbicides in river water by solid-phase-extraction high performance liquid chromatography with photoinduced-fluorimetric detection.

机构信息

Department of Analytical Chemistry, Faculty of Sciences, University of Extremadura, 06071 Badajoz, Spain.

出版信息

Talanta. 2003 Jun 13;60(2-3):279-85. doi: 10.1016/S0039-9140(03)00072-9.

Abstract

The separation and determination of five herbicides, including propanil and the phenylureas diuron, isoproturon, linuron and neburon, has been performed by an HPLC method, using photochemically-induced fluorescence detection. The non-fluorescent herbicides were transformed into fluorescent compounds by post-column photochemical reaction. A 60:40 (v/v) acetonitrile-buffer solution of potassium phosphate dibasic (pH 7, 0.01 M) was used for the chromatographic elution to separate propanil, linuron and neburon. The overlapping of isoproturon and diuron peaks, in the selected conditions, was resolved by changing the initial movil phase composition to 50:50 (v/v) methanol-buffer solution of potassium phosphate dibasic (pH 7, 0.01 M). The procedure was applied with satisfactory results to the analysis of these herbicides in Guadiana river water samples (Badajoz, Spain), allowing the detection of herbicide residues in the order of mug l(-1), by using a solid-phase extraction (SPE) pre-concentration step.

摘要

采用 HPLC 法，通过光化学诱导荧光检测，对五种除草剂，包括丙草胺和苯脲类除草剂敌草隆、异丙隆、利谷隆和灭草隆进行了分离和测定。非荧光除草剂通过柱后光化学反应转化为荧光化合物。用 60:40（v/v）乙腈-磷酸二氢钾缓冲液（pH7，0.01M）作为色谱洗脱液，可分离丙草胺、利谷隆和灭草隆。在选定的条件下，通过改变初始流动相组成，将 50:50（v/v）甲醇-磷酸二氢钾缓冲液（pH7，0.01M）用于分离异丙隆和敌草隆的重叠峰。该方法应用于瓜迪亚纳河水样（西班牙巴达霍斯）中这些除草剂的分析，结果令人满意，通过固相萃取（SPE）预浓缩步骤，可检测到微克/升（μg/L）级别的除草剂残留。