Department of Chemistry, Shanxi University, Taiyuan 030006, China.
Talanta. 2003 Jun 13;60(2-3):555-62. doi: 10.1016/S0039-9140(03)00185-1.
Oxygen is the most effective dynamic quencher in liquid room temperature phosphorimetry (LRTP). Using oxygen scavenger to achieve significant or more sensitive RTP signals may bring about kinds of trouble in many cases and is apparently not very convenient in procedures. To date a few examples on LRTP without deoxygenation have been reported. Herein, we present a new named anti-oxygen-quenching RTP system using deoxycholate as a rigid medium. It can induce both lipophilic alpha-bromonaphthalene or 1-bromo-2-methylnaphthalene (BrMeN) and strongly water-soluble meso-tetrakis-(4-trimethylaminophenyl)porphyrin palladium (Pd-TAPP) to produce strong RTP emission under sodium deoxycholate of 4 mmol l(-1) and incubation time of half an hour. The maximum excitation and emission wavelengths respectively lie at 408 and 680 nm, with phosphorescence lifetime of 0.39 ms for Pd-TAPP, and at 285 and 515 nm, with lifetime of 4.9 ms for BrMeN. It is supposed that the hydrophobic and oxyphobic sandwich structure formed by two deoxycholate molecules with a phosphor in the case of deoxycholate or the interdiction fully of oxygen molecule diffusion into the microenvironment where the phosphor exists in the case of cyclodextrin is a key factor in inducing anti-oxygen-quenching RTP.
氧是液体室温磷光法（LRTP）中最有效的动态猝灭剂。使用除氧剂来实现显著或更灵敏的 RTP 信号，在许多情况下可能会带来各种麻烦，而且在程序上显然不太方便。迄今为止，已经有一些关于无需除氧的 LRTP 的例子被报道。在此，我们提出了一种使用脱氧胆酸钠作为刚性介质的新型抗氧猝灭 RTP 体系。它可以诱导亲脂性的α-溴萘或 1-溴-2-甲基萘（BrMeN）以及水溶性很强的meso-四（4-三甲氨基苯基）卟啉钯（Pd-TAPP）在 4mmol/L 的脱氧胆酸钠和半小时的孵育时间下产生强烈的 RTP 发射。最大激发和发射波长分别为 408nm 和 680nm，Pd-TAPP 的磷光寿命为 0.39ms，最大激发和发射波长分别为 285nm 和 515nm，BrMeN 的磷光寿命为 4.9ms。据推测，在脱氧胆酸钠的情况下，两个脱氧胆酸钠分子形成的疏水性和疏氧性夹心结构，或者在环糊精的情况下完全阻止氧分子扩散到磷光体存在的微环境中，是诱导抗氧猝灭 RTP 的关键因素。
