Sabarudin Akhmad, Oshima Mitsuko, Ishii Naoe, Motomizu Shoji
Department of Chemistry, Faculty of Science, Okayama University, 3-1-1 Tsushimanaka, Okayama, Japan.
Talanta. 2003 Aug 29;60(6):1277-85. doi: 10.1016/S0039-9140(03)00234-0.
A highly sensitive fluorescence quenching method for the determination of silicate based on the formation of an ion associate between molybdosilicate and Rhodamine B (RB) in nitric acid medium was developed. A flow injection system coupled with a fluorescence detector was used for the measurement of fluorescence intensity at 560 and 580 nm as excitation and emission wavelengths, respectively. The calibration graph for Si showed a linear range of 0.1-5 ng cm(-3) with correlation coefficient of 0.9999, and the detection limit of 0.06 ng cm(-3). The proposed method was successfully applied to the determination of silicate in ultrapurified water with satisfactory results.
建立了一种高灵敏度荧光猝灭法，用于在硝酸介质中基于钼硅酸盐与罗丹明B（RB）形成离子缔合物来测定硅酸盐。采用流动注射系统与荧光检测器联用，分别以560和580 nm作为激发波长和发射波长来测量荧光强度。硅的校准曲线线性范围为0.1 - 5 ng cm⁻³，相关系数为0.9999，检测限为0.06 ng cm⁻³。该方法成功应用于超纯水中硅酸盐的测定，结果令人满意。
