新型流动注射荧光法测定痕量硅酸盐及其在超纯水分析中的应用。

Novel flow injection-fluorometric method for the determination of trace silicate and its application to ultrapurified water analysis.

作者信息

Sabarudin Akhmad, Oshima Mitsuko, Ishii Naoe, Motomizu Shoji

机构信息

Department of Chemistry, Faculty of Science, Okayama University, 3-1-1 Tsushimanaka, Okayama, Japan.

出版信息

Talanta. 2003 Aug 29;60(6):1277-85. doi: 10.1016/S0039-9140(03)00234-0.

DOI:10.1016/S0039-9140(03)00234-0

PMID:18969155

Abstract

A highly sensitive fluorescence quenching method for the determination of silicate based on the formation of an ion associate between molybdosilicate and Rhodamine B (RB) in nitric acid medium was developed. A flow injection system coupled with a fluorescence detector was used for the measurement of fluorescence intensity at 560 and 580 nm as excitation and emission wavelengths, respectively. The calibration graph for Si showed a linear range of 0.1-5 ng cm(-3) with correlation coefficient of 0.9999, and the detection limit of 0.06 ng cm(-3). The proposed method was successfully applied to the determination of silicate in ultrapurified water with satisfactory results.

摘要

建立了一种高灵敏度荧光猝灭法，用于在硝酸介质中基于钼硅酸盐与罗丹明B（RB）形成离子缔合物来测定硅酸盐。采用流动注射系统与荧光检测器联用，分别以560和580 nm作为激发波长和发射波长来测量荧光强度。硅的校准曲线线性范围为0.1 - 5 ng cm⁻³，相关系数为0.9999，检测限为0.06 ng cm⁻³。该方法成功应用于超纯水中硅酸盐的测定，结果令人满意。