Taniai Tetsuyuki, Sukegawa Masaaki, Sakuragawa Akio, Uzawa Atsushi
Department of Chemistry, Chiba Institute of Technology, 2-1-1, Shibazono, Narashino, Chiba 275-0023, Japan.
Talanta. 2003 Dec 23;61(6):905-12. doi: 10.1016/S0039-9140(03)00382-5.
In this paper, we describe an automated flow injection system for measuring the concentration of phosphate based on a fluorescence quenching reaction between Rhoadamine 6G and phosphomolybdate with a preconcentration column, which was packed with a molybdate-form ion exchange resin to collect and preconcentrate the phosphate in the sample solution. Rhodamine 6G was chosen because the reaction with phosphomolybdate was fast and did not require heat. For the construction of a stable flow injection system, an aqueous methanol solution was used as the cleaning reagent to overcome the precipitation of ion-associated complexes between Rhodamine 6G and phosphomolybdate or Rhodamine 6G and molybdate. For the preconcentration and collection of the phosphate ions in a water sample, a preconcentration column, which was packed with a molybdate-form ion exchange resin, was combined with the proposed flow injection system and applied to natural water samples containing low concentrations of phosphate.
在本文中，我们描述了一种基于罗丹明6G与磷钼酸盐之间的荧光猝灭反应、带有预浓缩柱的自动化流动注射系统，用于测定磷酸盐浓度。预浓缩柱填充有钼酸盐形式的离子交换树脂，用于收集和预浓缩样品溶液中的磷酸盐。选择罗丹明6G是因为它与磷钼酸盐的反应快速且无需加热。为构建稳定的流动注射系统，使用甲醇水溶液作为清洗试剂，以克服罗丹明6G与磷钼酸盐或罗丹明6G与钼酸盐之间离子缔合络合物的沉淀。为了预浓缩和收集水样中的磷酸根离子，将填充有钼酸盐形式离子交换树脂的预浓缩柱与所提出的流动注射系统相结合，并应用于含有低浓度磷酸盐的天然水样。
