基于点滴试验的分光光度流动注射法测定植物消化液中的锌
Spectrophotometric flow-injection determination of zinc in plant digests based on a spot test.
作者信息
Dias Ana Cristi B, Carneiro Josiane M T, Zagatto Elias A G
机构信息
Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, P.O. Box 96, Piracicaba, SP 13400-970, Brazil.
出版信息
Talanta. 2004 May 28;63(2):245-50. doi: 10.1016/j.talanta.2003.10.040.
A spot test was implemented in a flow-injection system for the spectrophotometric determination of zinc in digests of plant materials. It is based on the influence of Zn(2+) on the oxidation rate of 1-naphthylethylenediamine (NED) by hexacyanoferrate(III) under acidic conditions. In order to control the precipitate formation and to maintain the resulting suspension, a micellar medium was established by adding Triton X-100. The proposed system handles about 65 samples per hour, meaning 72mug NED and 9.0mg K(3)[Fe(CN)(6)] per determination. Baseline drift is usually <0.01 absorbance per hour and the analytical signals for 0.5-2.5mgl(-1) Zn range within ca. 0.07-0.45 absorbance. Linearity of the analytical curve is fair (r>0.999, n=6) and detection limit was estimated as 0.2mgl(-1) Zn. Results are precise (R.S.D.<1%, n=10) and in agreement with flame atomic absorption spectrometry and with certified values of standard reference materials.
在流动注射系统中进行了斑点试验，用于分光光度法测定植物材料消化液中的锌。它基于酸性条件下Zn(2+)对六氰合铁(III)氧化1-萘乙二胺（NED）速率的影响。为了控制沉淀形成并维持所得悬浮液，通过添加 Triton X-100 建立了胶束介质。所提出的系统每小时可处理约65个样品，即每次测定需要72μg NED和9.0mg K(3)[Fe(CN)(6)]。基线漂移通常每小时<0.01吸光度，0.5 - 2.5mg l(-1) Zn范围内的分析信号在约0.07 - 0.45吸光度之间。分析曲线的线性良好（r>0.999，n = 6），检测限估计为0.2mg l(-1) Zn。结果精确（R.S.D.<1%，n = 10），与火焰原子吸收光谱法以及标准参考物质的认证值一致。
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