Amini Mohammad K, Momeni-Isfahani Tahereh, Khorasani Jafar H, Pourhossein Mohammad
Chemistry Department, University of Isfahan, Isfahan 81746-73441, Iran.
Talanta. 2004 Jun 17;63(3):713-20. doi: 10.1016/j.talanta.2003.12.032.
An optical chemical sensor based on immobilization of 2-(5-bromo-2-pyridylazo)-5-(diethylamino)phenol (Br-PADAP) in Nafion membrane is described. The membranes were cast onto glass substrates and were used for the determination of nickel in aqueous solutions by spectrophotometry. The sensor system is highly transparent, mechanically stable and showed no evidence of reagent leaching. The influence of several parameters such as pH, ligand concentration, and type and concentration of regenerating solution were optimized. The sensor system showed good sensitivity in the range 0.5-20mugml(-1) with a detection limit of 0.3mugml(-1) Ni(II). The sensor has been incorporated into a home-made flow-through cell for determination of nickel in flowing streams with improved sensitivity, precision and detection limit. The calibration curve in the flow system was linear in the range 0.1-16mugml(-1) with a detection limit of 0.07mugml(-1). The sensor is easily regenerated by dilute nitric acid solution. The proposed method was successfully applied to the determination of nickel content in vegetable oil and chocolate samples and the results were compared with those obtained using atomic absorption spectrometry.
描述了一种基于将2-(5-溴-2-吡啶偶氮)-5-(二乙氨基)苯酚(Br-PADAP)固定在Nafion膜中的光学化学传感器。将该膜浇铸在玻璃基板上，并用于通过分光光度法测定水溶液中的镍。该传感器系统高度透明，机械稳定，且未显示出试剂浸出的迹象。优化了几个参数的影响，如pH值、配体浓度以及再生溶液的类型和浓度。该传感器系统在0.5-20μg/ml(-1)范围内显示出良好的灵敏度，镍(II)的检测限为0.3μg/ml(-1)。该传感器已被集成到自制的流通池中，用于测定流动样品中的镍，提高了灵敏度、精密度和检测限。流动系统中的校准曲线在0.1-16μg/ml(-1)范围内呈线性，检测限为0.07μg/ml(-1)。该传感器很容易用稀硝酸溶液再生。所提出的方法成功地应用于植物油和巧克力样品中镍含量的测定，并将结果与使用原子吸收光谱法获得的结果进行了比较。
