凝血酶对培养的内皮细胞产生糖胺聚糖的影响。
Effect of thrombin on the production of glycosaminoglycans by cultured endothelial cells.
作者信息
Kaji T, Akai T, Hayashi T, Sakuragawa N
机构信息
Department of Environmental Science, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan.
出版信息
Thromb Res. 1991 Jun 1;62(5):509-17. doi: 10.1016/0049-3848(91)90024-q.
We investigated the effect of thrombin on the production process of glycosaminoglycans (GAG) in cultured bovine aortic endothelial cells. It was revealed that the trichloroacetic acid-insoluble [35S]sulfate-labeled GAG (35S-GAG) was decreased by thrombin. The thrombin-induced decrease in the accumulation of 35S-GAG in the cell layer occurred even in the presence of actinomycin D or cycloheximide. The incorporation of both [35S]sulfate and [3H]glucosamine into the GAG was decreased by thrombin, while the ratio of 35S to 3H was not changed. Also, the incorporation of both [3H]glucosamine and [14C]UDP-xylose into the GAG was inhibited by thrombin. From these results, it was suggested that thrombin decreased GAG in the endothelial cell layer through an suppression of formation of polysaccharide chains rather than that of core protein.
我们研究了凝血酶对培养的牛主动脉内皮细胞中糖胺聚糖（GAG）产生过程的影响。结果显示，凝血酶可使三氯乙酸不溶性[35S]硫酸盐标记的GAG（35S-GAG）减少。即使存在放线菌素D或环己酰亚胺，凝血酶诱导的细胞层中35S-GAG积累的减少仍会发生。凝血酶使[35S]硫酸盐和[3H]葡糖胺掺入GAG的量均减少，而35S与3H的比例未发生变化。此外，凝血酶还抑制了[3H]葡糖胺和[14C]UDP-木糖掺入GAG。从这些结果表明，凝血酶通过抑制多糖链的形成而非核心蛋白的形成来减少内皮细胞层中的GAG。
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