在玻璃电泳芯片上集成流动型化学发光检测器。

Integration of a flow-type chemiluminescence detector on a glass electrophoresis chip.

作者信息

Su Rongguo, Lin Jin-Ming, Uchiyama Katsumi, Yamada Masaaki

机构信息

Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing 100085, China.

出版信息

Talanta. 2004 Nov 15;64(4):1024-9. doi: 10.1016/j.talanta.2004.04.024.

DOI:10.1016/j.talanta.2004.04.024

PMID:18969706

Abstract

A glass electrophoresis microchip integrated a flow-type chemiluminescence (CL) detection cell has been developed and evaluated. The chip pattern is a double-T-type electrophoretic sample injection and separation combining with a Y-type chemiluminecent detector. The double-T geometry allows for high-efficiency sample injection and geometric definition of sample plug size. The branch of Y was used as CL reagent channel, and the CL reagent was delivered by a lab-made micropump. Bis[(2,4,6-trichlorophenyl)]oxalate-H(2)O(2) CL system was employed to detect dansyl amino acids. On this microchip, dansyl-phenylalanine and -sarcosine were successfully separated by electrophoresis and detected within 250s. The detection limits (S/N=3) of dansyl-phenylalanine and -sarcosine could reach to 2.8 and 3.2muM, respectively, due to the vigorous dilution of sample with CL reagent and timely removal of the waste solution from reaction area.

摘要

一种集成了流动式化学发光（CL）检测池的玻璃电泳微芯片已被开发并评估。芯片图案为双T型电泳进样和分离与Y型化学发光检测器相结合。双T型结构允许高效进样以及对样品塞大小进行几何定义。Y型分支用作CL试剂通道，CL试剂由自制的微型泵输送。采用双（2,4,6 - 三氯苯基）草酸酯 - H₂O₂ CL体系检测丹磺酰氨基酸。在该微芯片上，丹磺酰苯丙氨酸和丹磺酰肌氨酸通过电泳成功分离，并在250秒内完成检测。由于样品被CL试剂剧烈稀释且反应区域的废液被及时清除，丹磺酰苯丙氨酸和丹磺酰肌氨酸的检测限（S/N = 3）分别可达2.8和3.2μM。