高效液相色谱法同时测定全血中环孢素A及其四种主要代谢物

High-performance liquid chromatographic method for the simultaneous determination of cyclosporine A and its four major metabolites in whole blood.

作者信息

Khoschsorur G, Erwa W, Fruehwirth F, Stettin M, Meinitzer A, Hoebarth G, Zelzer S, Halwachs-Baumann G

机构信息

Institute of Medical and Chemical Laboratory Diagnostics, University Medical Center, Auenbruggerplatz 29, Graz A-8036, Austria.

出版信息

Talanta. 2005 Feb 15;65(3):638-43. doi: 10.1016/j.talanta.2004.07.048.

DOI:10.1016/j.talanta.2004.07.048

PMID:18969847

Abstract

This study aimed to develop a simple and efficient optimized high-performance liquid chromatograph (HPLC) method for simultaneous determination of cyclosporine A (CyA) and its major, partly active metabolites AM1, AM9, AM4N, and AM19 in whole blood from transplant patients using cyclosporine D (CyD) as internal standard. The method used a CN analytical column maintained at 60 degrees C with hexan-isopropanol (93:7, v/v) as mobile phase; detection was at 212nm. Linearity for all five compounds was tested in the range of 31-1500ngml(-1) for CyA and of 31-1000ngml(-1) for metabolites. The limit of detection was found to be 15ngml(-1) for all compounds. This modified, inexpensive method is also suitable for measuring cyclosporine A and metabolite concentrations in routine monitoring of patients undergoing treatment with CyA.

摘要

本研究旨在开发一种简单高效的优化高效液相色谱（HPLC）方法，以环孢素D（CyD）为内标，同时测定移植患者全血中环孢素A（CyA）及其主要的部分活性代谢物AM1、AM9、AM4N和AM19。该方法采用CN分析柱，柱温保持在60℃，以正己烷-异丙醇（93:7，v/v）为流动相；检测波长为212nm。对所有五种化合物进行线性测试，CyA的线性范围为31 - 1500ng/ml(-1)，代谢物的线性范围为31 - 1000ng/ml(-1)。所有化合物的检测限均为15ng/ml(-1)。这种经过改进的廉价方法也适用于在接受CyA治疗的患者的常规监测中测量环孢素A和代谢物的浓度。