Mahedero M C, Díaz N Mora, Muñoz de la Peña A, Espinosa Mansilla A, Gónzalez Gómez D, Bohoyo Gil D
Department of Analytical Chemistry, University of Extremadura, 06071 Badajoz, Spain.
Talanta. 2005 Feb 15;65(3):806-13. doi: 10.1016/j.talanta.2004.08.055.
A widely employed compound for honey treatment, sulfathiazole (ST), was determined in commercial honey samples, employing a combination of photochemically induced fluorescence excitation-emission matrices (EEMs) and chemometric processing of the recorded second-order data. Parallel Factor Analysis (PARAFAC) and Self-Weighted Alternating Trilinear Decomposition (SWATLD) methods were used for calibration. An appropriately designed calibration with a set of standards composed of 18 samples, coupled to the use of the second-order advantage offered by the applied chemometric techniques, allowed quantitation of sulfathiazole in spiked commercial honey samples. No previous separation or sample pretreatment steps were required. The results were compared with other calibration methods such as N-PLS and PLS-1 that produced good results on synthetic samples but not on the investigated commercial honey samples.
采用光化学诱导荧光激发-发射矩阵（EEMs）与记录的二阶数据化学计量学处理相结合的方法，测定了市售蜂蜜样品中一种广泛用于蜂蜜处理的化合物——磺胺噻唑（ST）。使用平行因子分析（PARAFAC）和自加权交替三线性分解（SWATLD）方法进行校准。通过由18个样品组成的一组标准品进行适当设计的校准，并结合所应用化学计量学技术提供的二阶优势，能够对加标市售蜂蜜样品中的磺胺噻唑进行定量。无需先前的分离或样品预处理步骤。将结果与其他校准方法（如N-PLS和PLS-1）进行比较，这些方法在合成样品上取得了良好结果，但在研究的市售蜂蜜样品上效果不佳。
