Silva Claudineia R, Vieira Heberth J, Canaes Larissa S, Nóbrega Joaquim A, Fatibello-Filho Orlando
Laboratório de Analítica, Bionalítica, Biossensores, Eletroanalítica e Sensores, Departamento de Química, Universidade Federal de São Carlos, Caixa Postal 676, CEP 13560-970 São Carlos, SP, Brazil.
Talanta. 2005 Feb 28;65(4):965-70. doi: 10.1016/j.talanta.2004.08.028.
A flow injection (FI) spectrophotometric method was proposed for the determination of chloride ion in natural waters. The determination of chloride was carried out by reaction with Hg(SCN)(2) immobilized in an epoxy resin bead in a solid-phase reactor (SPR) and the thiocyanate ions released were determined spectrophotometrically at 480nm after complexing reaction with Fe(III). The analytical curve for chloride was linear in the concentration range from 5.6 x 10(-5) to 2.2 x 10(-4)moll(-1) with a detection limit of 1.4 x 10(-5)moll(-1). The relative standard deviation (R.S.D.) was 2.2% for a solution containing 2.2 x 10(-4)moll(-1) (n = 10). The simple manifold allows a routine analytical frequency of 100 determinations per hour. The main advantage of the developed method is the 400% reduction of the Hg waste solution generated when compared to conventional methods for chloride determination based on the same spectrophotometric reaction.
提出了一种流动注射（FI）分光光度法用于测定天然水中的氯离子。通过与固定在固相反应器（SPR）中环氧树脂珠粒上的Hg(SCN)₂反应来测定氯离子，释放出的硫氰酸根离子在与Fe(III)络合反应后于480nm处进行分光光度测定。氯离子的分析曲线在5.6×10⁻⁵至2.2×10⁻⁴mol·l⁻¹的浓度范围内呈线性，检测限为1.4×10⁻⁵mol·l⁻¹。对于含有2.2×10⁻⁴mol·l⁻¹的溶液（n = 10），相对标准偏差（R.S.D.）为2.2%。简单的流路使得常规分析频率可达每小时100次测定。所开发方法的主要优点是与基于相同分光光度反应的传统氯离子测定方法相比，汞废液的产生量减少了400%。
