Andreu Yolanda, de Marcos Susana, Castillo Juan R, Galbán Javier
Analytical Spectroscopy and Sensors Group (GEAS), Analytical Chemistry Department, Science Faculty, University of Zaragoza, Zaragoza 50009, Spain.
Talanta. 2005 Feb 28;65(4):1045-51. doi: 10.1016/j.talanta.2004.08.036.
An analytical method based on the absorption changes of chemically polymerised polyaniline at 700nm is proposed for the determination of Vitamin C. Vitamin C produces a polyaniline film reduction, originating changes in its absorbance proportional to the Vitamin C concentration. The optimum reaction conditions and the analytical characteristics have been studied. The linear response of the method ranged from 0.10 to 1.0mgl(-1) for a 6min reaction time and from 1.0 to 8.0mgl(-1) for a 2min reaction time. Reproducibility, expressed as the coefficient of variation, was 0.8% (6min reaction time) and 2.3% (2min reaction time) (n = 10). The method has been applied to Vitamin C determination in pharmaceutical preparations and commercial fruit juices. The results were compared with those obtained by the 2,6-dichlorophenolindophenol titration method (the AOAC Official Method) and no systematic errors were observed.
提出了一种基于化学聚合聚苯胺在700nm处吸收变化的分析方法用于测定维生素C。维生素C会使聚苯胺膜还原，导致其吸光度发生变化，该变化与维生素C浓度成正比。研究了最佳反应条件和分析特性。该方法在反应时间为6分钟时，线性响应范围为0.10至1.0mg/L；反应时间为2分钟时，线性响应范围为1.0至8.0mg/L。以变异系数表示的重现性在反应时间为6分钟时为0.8%，反应时间为2分钟时为2.3%（n = 10）。该方法已应用于药物制剂和市售果汁中维生素C的测定。将结果与通过2,6 - 二氯酚靛酚滴定法（AOAC官方方法）获得的结果进行比较，未观察到系统误差。
