Kang Jianzhen, Yan Jilin, Liu Jifeng, Qiu Haibo, Yin Xue-Bo, Yang Xiurong, Wang Erkang
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
Talanta. 2005 May 15;66(4):1018-24. doi: 10.1016/j.talanta.2005.01.002.
In this paper a method was described about dynamic coating for resolving rhodamine B (RB) adsorption on a hybrid poly(dimethylsiloxane) (PDMS)/glass chip. The results showed that when the non-ionic surfactant Triton X-100 was higher than 0.5% (v/v) into the phosphate buffer, the adsorption of RB appeared. Besides, some separation conditions for RB were investigated, including concentration of Triton X-100, concentration and pH value of running buffer, separation voltage and detection site. Through comparing electroosmotic flow, plate numbers and other parameters, an acceptable separation condition was obtained. Under optimized conditions, the precisions of RB detection (R.S.D., n=10) were 2.62% for migration time, 4.78% for peak height respectively. Additionally, RB concentration linearity response was excellent with 0.9996 of correlation coefficient between 1 and 100muM, and a limit of detection (S/N=3) was 0.2muM. Finally, we separated rhodamine B isothiocyanate and lysine deriving from the fluorescent probe, and the result displayed that the dynamic coating method was applicable by CE separations using PDMS/glass chip.
本文描述了一种用于解决若丹明B（RB）在聚二甲基硅氧烷（PDMS）/玻璃混合芯片上吸附问题的动态涂层方法。结果表明，当磷酸盐缓冲液中加入的非离子表面活性剂Triton X-100高于0.5%（v/v）时，会出现RB的吸附现象。此外，还研究了RB的一些分离条件，包括Triton X-100的浓度、运行缓冲液的浓度和pH值、分离电压和检测位点。通过比较电渗流、塔板数等参数，获得了可接受的分离条件。在优化条件下，RB检测的精密度（R.S.D.，n = 10）迁移时间为2.62%，峰高为4.78%。此外，RB浓度线性响应良好，在1至100μM之间相关系数为0.9996，检测限（S/N = 3）为0.2μM。最后，我们分离了异硫氰酸若丹明B和荧光探针衍生的赖氨酸，结果表明动态涂层方法适用于使用PDMS/玻璃芯片的毛细管电泳分离。
