铋膜电极上差分脉冲阳极溶出伏安法检测金属硫蛋白
Differential pulse anodic stripping voltammetry detection of metallothionein at bismuth film electrodes.
作者信息
Yang Minli, Zhang Zhanjun, Hu Zhongbo, Li Jinghong
机构信息
Department of Chemistry, Key Lab. of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing, China.
出版信息
Talanta. 2006 Jul 15;69(5):1162-5. doi: 10.1016/j.talanta.2005.12.041. Epub 2006 Jan 19.
As a representation of metalloproteins, metallothionein (MT), which plays important biological and environmental roles such as in the metabolism and detoxification of some metals, was detected at bismuth film electrode (BiFE) by differential pulse anodic stripping voltammetry (DPASV). In pH 2-5.5, two well-defined anodic peaks were produced and attributed to the Zn(2+) and Cd(2+) inherent to MT. The calibration plot of DPASV peak currents for Cd(2+) inherent to MT versus MT concentrations showed a good linearity with a detection limit of 3.86 x 10(-8)mol/L for MT. As a non-toxic excellent electrode material, BiFE shows good performance for detecting MT, and is expected to find further applications in the studies of many other metalloproteins.
作为金属蛋白的一种代表，金属硫蛋白（MT）在某些金属的代谢和解毒等生物和环境过程中发挥着重要作用，通过差分脉冲阳极溶出伏安法（DPASV）在铋膜电极（BiFE）上对其进行了检测。在pH值为2 - 5.5的条件下，产生了两个清晰的阳极峰，它们归因于MT中固有的Zn(2+)和Cd(2+)。MT中Cd(2+)的DPASV峰电流与MT浓度的校准曲线显示出良好的线性关系，MT的检测限为3.86×10(-8)mol/L。作为一种无毒的优良电极材料，BiFE在检测MT方面表现出良好的性能，有望在许多其他金属蛋白的研究中得到进一步应用。
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