Fang Dong-Mei, Wu Hai-Long, Ding Yu-Jie, Hu Le-Qian, Xia A-Lin, Yu Ru-Qin
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
Talanta. 2006 Aug 15;70(1):58-62. doi: 10.1016/j.talanta.2006.01.014. Epub 2006 Feb 21.
Fluoroquinolones or so-called second-generation quinolones, in particular, ofloxacin (OFL), norfloxacin (NOR), and enoxacin (ENO), with therapeutic advantages possess strongly overlapped fluorescence spectra. In this paper, two strategies were proposed for simultaneous direct determination of OFL, NOR and ENO in plasma by combining fluorescence excitation-emission matrix (EEM) with second-order calibration based on the alternating trilinear decomposition algorithm (ATLD) and parallel factor analysis (PARAFAC). The results showed that both algorithms could solve the problem of serious fluorescence spectral overlapping of the sought-for analytes even in the presence of uncalibrated interferents. However, ATLD has advantages of being insensitive to overestimated component number and fast convergence. The results by using ATLD with an estimated component number of five were reasonably acceptable for clinical analysis. The average recoveries of OFL, NOR and ENO in synthetic samples were 99.7+/-2.4, 101.5+/-2.4 and 97.3+/-3.8%, respectively; the average recoveries of OFL, NOR and ENO in complex plasma were 94.3+/-2.6, 85.6+/-3.3 and 103.3+/-3.0%, respectively.
氟喹诺酮类药物，即所谓的第二代喹诺酮类药物，特别是氧氟沙星（OFL）、诺氟沙星（NOR）和依诺沙星（ENO），具有治疗优势，但荧光光谱严重重叠。本文提出了两种策略，通过将荧光激发 - 发射矩阵（EEM）与基于交替三线性分解算法（ATLD）和平行因子分析（PARAFAC）的二阶校正相结合，同时直接测定血浆中的OFL、NOR和ENO。结果表明，即使存在未校准的干扰物，两种算法都能解决目标分析物荧光光谱严重重叠的问题。然而，ATLD具有对高估组分数不敏感和收敛速度快的优点。使用估计组分数为五的ATLD得到的结果对于临床分析来说是合理可接受的。合成样品中OFL、NOR和ENO的平均回收率分别为99.7±2.4%、101.5±2.4%和97.3±3.8%；复杂血浆中OFL、NOR和ENO的平均回收率分别为94.3±2.6%、85.6±3.3%和103.3±3.0%。
