Zuo Yuegang, Wang Chengjun, Van Thuan
Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, 285 Old Westport Road, North Dartmouth, MA 02747, United States; University of Massachusetts, Graduate School of Marine Sciences and Technology, 285 Old Westport Road, North Dartmouth, MA 02747, United States.
Talanta. 2006 Sep 15;70(2):281-5. doi: 10.1016/j.talanta.2006.02.034. Epub 2006 Mar 15.
A simple, fast, sensitive and accurate reversed-phase ion-pair HPLC method for simultaneous determination of nitrite and nitrate in atmospheric liquids and lake waters has been developed. Separations were accomplished in less than 10min using a reversed-phase C(18) column (150mmx2.00mm i.d., 5mum particle size) with a mobile phase containing 83% 3.0mM ion-interaction reagent tetrabutylammonium hydroxide (TBA-OH) and 2.0mM sodium phosphate buffer at pH 3.9 and 17% acetonitrile (flow rate, 0.4mL/min). UV light absorption responses at 205nm were linear over a wide concentration range from 100mug/mL to the detection limits of 10mug/L for nitrite and 5mug/L nitrate. Quantitation was carried out by the peak area method. The relative standard deviation for the analysis of nitrite and nitrate was less than 3.0%. This method was applied for the simultaneous determination of nitrite and nitrate in dew, rain, snow and lake water samples collected in southeast Massachusetts. Nitrate was found being present at 4.79-5.99mug/mL in dew, 1.20-2.63mug/mL in rain, 0.32-0.60mug/mL in snow and 0.12-0.23mug/mL in lake water. Nitrite was only a minor species in dew (0.62-0.83mug/mL), rain (<0.005-0.14mug/mL), snow (0.021-0.032mug/mL) and lake water (0.12-0.16mug/mL). High levels of nitrite and nitrate observed in dew water droplets may constitute an important source of hydroxyl radicals in the sunny early morning.
已开发出一种简单、快速、灵敏且准确的反相离子对高效液相色谱法，用于同时测定大气液体和湖水中的亚硝酸盐和硝酸盐。使用反相C(18)柱（内径150mm×2.00mm，粒径5μm），在不到10分钟内完成分离，流动相包含83%的3.0mM离子相互作用试剂氢氧化四丁基铵（TBA-OH）和2.0mM pH 3.9的磷酸钠缓冲液以及17%的乙腈（流速0.4mL/min）。在205nm处的紫外光吸收响应在100μg/mL至亚硝酸盐10μg/L和硝酸盐5μg/L的检测限的宽浓度范围内呈线性。通过峰面积法进行定量。亚硝酸盐和硝酸盐分析的相对标准偏差小于3.0%。该方法用于同时测定在马萨诸塞州东南部采集的露水、雨水、雪水和湖水样品中的亚硝酸盐和硝酸盐。发现露水中硝酸盐含量为4.79 - 5.99μg/mL，雨水中为1.20 - 2.63μg/mL，雪水中为0.32 - 0.60μg/mL，湖水中为0.12 - 0.23μg/mL。亚硝酸盐在露水（0.62 - 0.83μg/mL）、雨水（<0.005 - 0.14μg/mL）、雪水（0.021 - 0.032μg/mL）和湖水中（0.12 - 0.16μg/mL）仅为少量成分。在阳光明媚的清晨，露水中观察到的高浓度亚硝酸盐和硝酸盐可能构成羟基自由基的重要来源。
