Zemberyová Mária, Barteková Jana, Hagarová Ingrid
Comenius University in Bratislava, Faculty of Natural Sciences, Department of Analytical Chemistry, Mlynská dolina CH-2, 842 15 Bratislava, Slovak Republic.
Talanta. 2006 Dec 15;70(5):973-8. doi: 10.1016/j.talanta.2006.05.057. Epub 2006 Jul 5.
A modified three-step sequential extraction procedure for the fractionation of heavy metals, proposed by the Commission of the European Communities Bureau of Reference (BCR) has been applied to the Slovak reference materials of soils (soil orthic luvisols, soil rendzina and soil eutric cambisol), which represent pedologically different types of soils in Slovakia. Analyses were carried out by flame or electrothermal atomic absorption spectrometry (FAAS or ETAAS). The fractions extracted were: exchangeable (extraction step 1), reducible-iron/manganese oxides (extraction step 2), oxidizable-organic matter and sulfides (extraction step 3). The sum of the element contents in the three fractions plus aqua-regia extractable content of the residue was compared to the aqua-regia extractable content of the elements in the origin soils. The accuracy obtained by comparing the determined contents of the elements with certified values, using BCR CRM 701, certified for the extractable contents (mass fractions) of Cd, Cr, Cu, Ni, Pb and Zn in sediment following a modified BCR-three step sequential extraction procedure, was found to be satisfactory.
欧洲共同体参考局（BCR）提出的一种用于重金属分级的改良三步连续萃取程序，已应用于斯洛伐克土壤参考物质（土壤雏形淋溶土、土壤腐殖质土和土壤普通雏形土），这些土壤代表了斯洛伐克不同土壤学类型的土壤。分析采用火焰或电热原子吸收光谱法（FAAS或ETAAS）进行。萃取的部分为：可交换态（萃取步骤1）、可还原态铁/锰氧化物（萃取步骤2）、可氧化态有机物和硫化物（萃取步骤3）。将这三个部分中元素含量的总和加上残渣中王水可萃取的含量，与原始土壤中元素的王水可萃取含量进行比较。使用经改良的BCR三步连续萃取程序对沉积物中镉、铬、铜、镍、铅和锌的可萃取含量（质量分数）进行认证的BCR CRM 701，将测定的元素含量与认证值进行比较，结果发现准确度令人满意。
