Matús Peter, Kubová Jana, Bujdos Marek, Medved' Ján
Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská dolina 1, 842 15 Bratislava, Slovakia.
Talanta. 2006 Dec 15;70(5):996-1005. doi: 10.1016/j.talanta.2006.05.045. Epub 2006 Jun 27.
The single extractions with 15 extractants (agents) (H(2)O, KCl, NH(4)Cl, NH(4)F, CaCl(2), BaCl(2), CuCl(2), LaCl(3), Na(2)S(2)O(4), (NH(4))(2)C(2)O(4), Na(4)P(2)O(7), NTA, EDTA, DTPA, HCl), the optimised BCR (Community Bureau of Reference) three-step sequential extraction procedure (SEP) and the solid phase extraction (SPE) by the chelating ion-exchanger Iontosorb Salicyl (cellulose resin containing covalently bound salicylic acid functional groups) were used for the partitioning of Al in very acid soil samples taken from an area influenced by acid mine solutions. The precision, accuracy and repeatibility for all steps of the optimised BCR SEP were checked on the various reference materials (CRM 483 sewage sludge amended soil, CRM BCR 701 freshwater sediment, SRM 2710 and SRM 2711 Montana soils). Also the new indicative values of the optimised BCR SEP fractional Al concentrations were obtained for these reference materials. The aluminium amounts obtained by the used extraction procedures were valuated and discussed from the aspect of the Al concentration in the plants (grass) growing on the same studied soils. The aluminium toxicity indexes (ATI) calculated for the studied soils, the BaCl(2) and acetic acid soil extracts and the grass stems and roots were used for the assessment of the Al toxicity to the plants. The ATI value was defined as the ratio of the nutrient cations (Ca, Mg, K, Na) concentration sum to the Al concentration. The flame atomic absorption spectrometry (LOQ=0.2mgl(-1)) and the inductively coupled plasma optical emission spectrometry (LOQ=0.03mgl(-1)) were used for the aluminium quantification.
采用15种萃取剂（试剂）（水、氯化钾、氯化铵、氟化铵、氯化钙、氯化钡、氯化铜、氯化镧、硫代硫酸钠、草酸铵、焦磷酸钠、氮川三乙酸、乙二胺四乙酸、二乙三胺五乙酸、盐酸）进行单次萃取，采用优化后的BCR（标准物质与测量研究所）三步连续萃取程序（SEP）以及通过螯合离子交换剂Iontosorb Salicyl（含有共价结合水杨酸官能团的纤维素树脂）进行固相萃取（SPE），对取自受酸性矿山溶液影响地区的强酸性土壤样品中的铝进行分配。在各种参考物质（CRM 483污水污泥改良土壤、CRM BCR 701淡水沉积物、SRM 2710和SRM 2711蒙大拿土壤）上检查了优化后的BCR SEP所有步骤的精密度、准确度和重复性。还获得了这些参考物质的优化后的BCR SEP分数铝浓度的新指示值。从生长在相同研究土壤上的植物（草）中的铝浓度方面对通过所用萃取程序获得的铝含量进行了评估和讨论。计算了所研究土壤、氯化钡和乙酸土壤提取物以及草茎和根的铝毒性指数（ATI），用于评估铝对植物的毒性。ATI值定义为营养阳离子（钙、镁、钾、钠）浓度总和与铝浓度的比值。采用火焰原子吸收光谱法（定量限=0.2mg l⁻¹）和电感耦合等离子体发射光谱法（定量限=0.03mg l⁻¹）进行铝的定量分析。
