Chen Chuan, Wang Aijie, Ren Nanqi, Kan Hongjing, Lee Duu-Jong
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, P.O. Box 2614, 202 Haihe Road, Harbin, 150090, China.
Appl Microbiol Biotechnol. 2008 Dec;81(4):765-70. doi: 10.1007/s00253-008-1720-y. Epub 2008 Oct 30.
This work conducted a denitrifying sulfide removal (DSR) test in an expanded granular sludge bed (EGSB) reactor at sustainable loadings of 6.09 kg m(-3) day(-1) for sulfide, 3.11 kg m(-3) day(-1) for nitrate-nitrogen, and 3.27 kg m(-1) day(-1) for acetate-carbon with >93% efficiency, which is significantly higher than those reported in literature. Strains Pseudomonas sp., Nitrincola sp., and Azoarcus sp. very likely yield heterotrophs. Strains Thermothrix sp. and Sulfurovum sp. are the autotrophs required for the proposed high-rate EGSB-DSR system. The EGSB-DSR reactor experienced two biological breakdowns, one at loadings of 4.87, 2.13, and 1.82 kg m(-3) day(-1); reactor function was restored by increasing nitrate and acetate loadings. Another breakdown occurred at loadings of up to 8.00, 4.08, and 4.50 kg m(-1) day(-1); the heterotrophic denitrification pathway declined faster than the autotrophic pathway. The mechanism of DSR breakdown is as follows. High sulfide concentration inhibits heterotrophic denitrifiers, and the system therefore accumulates nitrite. Autotrophic denitrifiers are then inhibited by the accumulated nitrite, thereby leading to breakdown of the DSR process.
本研究在膨胀颗粒污泥床（EGSB）反应器中进行了反硝化除硫（DSR）试验，硫化物的可持续负荷为6.09 kg m(-3) 天(-1)，硝酸盐氮为3.11 kg m(-3) 天(-1)，乙酸盐碳为3.27 kg m(-1) 天(-1)，去除效率>93%，显著高于文献报道。假单胞菌属、硝化螺菌属和偶氮弧菌属菌株很可能产生异养菌。嗜热丝菌属和硫卵菌属菌株是所提出的高速率EGSB-DSR系统所需的自养菌。EGSB-DSR反应器经历了两次生物故障，一次是在负荷为4.87、2.13和1.82 kg m(-3) 天(-1)时；通过增加硝酸盐和乙酸盐负荷恢复了反应器功能。另一次故障发生在负荷高达8.00、4.08和4.50 kg m(-1) 天(-1)时；异养反硝化途径比自养途径下降得更快。DSR故障的机制如下。高硫化物浓度抑制异养反硝化菌，因此系统积累亚硝酸盐。自养反硝化菌随后被积累的亚硝酸盐抑制，从而导致DSR过程的故障。
