Harvey Billie J, Levitus Marcia
Biodesign Institute, Arizona State University, Tempe, AZ 85287-5601, USA.
J Fluoresc. 2009 May;19(3):443-8. doi: 10.1007/s10895-008-0431-1. Epub 2008 Oct 30.
We report on the role of dye-nucleobase interactions on the photophysical properties of the indocarbocyanine Cy3. The fluorescence efficiency and lifetime ofCy3 increase in the presence of all four nucleoside monophosphates. This behavior correlates with an increase in the activation energy for photoisomerization and a approximately 4 nm red shift in the fluorescence spectrum. Changes are more dramatic for the purines (dAMP, dGMP) than the pyrimidines(dCMP, dTMP), and for the nonsulfonated cyanine(DiIC2(3)) than the sulfonated dye (Cy3-SE). These results are consistent with a model in which Cy3-nucleoside pi-pi interactions decrease the efficiency of photoisomerization,increasing the efficiency of fluorescence.
我们报道了染料-核碱基相互作用对吲哚碳菁Cy3光物理性质的影响。在所有四种核苷单磷酸存在的情况下，Cy3的荧光效率和寿命都会增加。这种行为与光异构化活化能的增加以及荧光光谱中约4nm的红移相关。嘌呤（dAMP、dGMP）比嘧啶（dCMP、dTMP）引起的变化更显著，非磺化花菁（DiIC2(3)）比磺化染料（Cy3-SE）引起的变化更显著。这些结果与一个模型一致，即Cy3-核苷π-π相互作用降低了光异构化效率，提高了荧光效率。
