Karsten Stanislav L, Kudo Lili C, Geschwind Daniel H
David Geffen School of Medicine at UCLA, Los Angeles, California, USA.
Curr Protoc Neurosci. 2008 Oct;Chapter 4:Unit 4.28. doi: 10.1002/0471142301.ns0428s45.
DNA microarrays pose specific challenges to those studying the central and peripheral nervous systems. Probably the most important involve difficulty in obtaining appropriate tissue for study, as well as the problems posed by cellular heterogeneity. This unit describes advances in the available technologies and provides protocols for cDNA microarray hybridization, including the use of PCR amplicons. Protocols are also provided for the two major methods for limiting cellular heterogeneity by study of RNA from single cell populations in high-throughput microarray studies, laser capture microdissection (LCM), and automated fluorescent cell sorting (FACS-array).
DNA微阵列给中枢神经系统和周围神经系统的研究者带来了特殊的挑战。可能最重要的挑战包括难以获取用于研究的合适组织，以及细胞异质性带来的问题。本单元描述了现有技术的进展，并提供了cDNA微阵列杂交的方案，包括PCR扩增子的使用。还提供了在高通量微阵列研究中通过研究单细胞群体的RNA来限制细胞异质性的两种主要方法的方案，即激光捕获显微切割（LCM）和自动荧光细胞分选（FACS阵列）。
