Pontin Jennifer, Blaylock Morgan G, Walsh Garry M, Turner Stephen W
Department of Child Health, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, UK.
School of Medicine, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, UK.
Pediatr Pulmonol. 2008 Nov;43(11):1130-1134. doi: 10.1002/ppul.20921.
Exhaled nitric oxide (FE(NO)), a potential biomarker for asthma, is positively correlated with eosinophilic airway inflammation. Eosinophil apoptotic rate (AR) may be increased by NO but the relationship between eosinophil AR and NO has not been studied in vivo. This study tested the hypothesis that eosinophil AR will be positively related to FE(NO).
Children with and without asthma were recruited and participated in an assessment that included FE(NO) measurement, skin prick reactivity, spirometry, and sputum induction. The absolute sputum eosinophil count and eosinophil AR were determined by morphology under light microscope after staining.
There were 31 children recruited, mean age 11 years, 21 were asthmatic and 19 were boys. The median FE(NO) (range) was 15.6 parts per billion (3.1-102.6), 17 were atopic and the mean (SD)% FEV(1) was 85 (10)%. Sputum eosinophil AR was determined in 19 children (16 asthmatics), mean (SD) value 0.49 (0.13). There were positive relationships between eosinophil AR and FE(NO) (Spearman rho = 0.46, P = 0.046), eosinophil AR and % eosinophil count (Spearman rho = 0.45, P = 0.050) and also FE(NO) and % eosinophil count (Spearman rho = 0.49, P = 0.024).
There is a positive relationship between FE(NO) and eosinophil AR. Nitric oxide may be involved in regulation of eosinophil AR in the airways.
呼出一氧化氮（FE(NO)）是哮喘的一种潜在生物标志物，与嗜酸性粒细胞气道炎症呈正相关。一氧化氮可能会提高嗜酸性粒细胞凋亡率（AR），但尚未在体内研究嗜酸性粒细胞AR与一氧化氮之间的关系。本研究检验了嗜酸性粒细胞AR与FE(NO)呈正相关的假设。
招募有或无哮喘的儿童，让他们参与一项评估，包括FE(NO)测量、皮肤点刺反应性、肺功能测定和痰液诱导。染色后，通过光学显微镜下的形态学方法测定痰液嗜酸性粒细胞绝对计数和嗜酸性粒细胞AR。
共招募了31名儿童，平均年龄11岁，其中21名患有哮喘，19名是男孩。FE(NO)的中位数（范围）为十亿分之15.6（3.1 - 102.6），17名儿童患有特应性疾病，FEV(1)的平均（标准差）百分比为85（10）%。对19名儿童（16名哮喘患者）测定了痰液嗜酸性粒细胞AR，平均（标准差）值为0.49（0.13）。嗜酸性粒细胞AR与FE(NO)之间存在正相关（斯皮尔曼等级相关系数rho = 0.46，P = 0.046），嗜酸性粒细胞AR与嗜酸性粒细胞计数百分比之间存在正相关（斯皮尔曼等级相关系数rho = 0.45，P = 0.050），FE(NO)与嗜酸性粒细胞计数百分比之间也存在正相关（斯皮尔曼等级相关系数rho = 0.49，P = 0.024）。
FE(NO)与嗜酸性粒细胞AR之间存在正相关。一氧化氮可能参与气道中嗜酸性粒细胞AR的调节。
