[Cx43对针刺原发性痛经大鼠效应的影响]

[Influence of Cx43 on acupuncture effect on the primary dysmenorrheal rat].

作者信息

Liu Fang, Zheng Cui-Hong, Huang Guang-Ying, Wang Wei

机构信息

Institute of Integrated Chinese and Western Medicine, Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.

出版信息

Zhongguo Zhen Jiu. 2008 Oct;28(10):751-6.

PMID:18972735

Abstract

OBJECTIVE

To observe the influence of silencing Connexin43 (Cx43) expression of partial acupoints on acupuncture effect, so as to probe into the mechanism of acupuncture treatment for primary dysmenorrhea.

METHODS

The primary dysmenorrheal rat model made by oxytocin and RNA interference (RNAi) technology was used to silence the expression of Cx43 in acupoints. Fifty SD female rats were divided into five groups, a normal group (N), a model group (M), an acupuncture group (A), an acupuncture plus interference group (A+I), an acupuncture plus interference control group (A+IC). RT-PCR method was used to observe the oxytocin receptor (OTR) and vasopressin receptor (VPR) mRNA expressions in the uterus in each group. Plasma prostaglandin E2 (PGE2) and PGF2alpha levels were detected by radioimmunoassay and ELISA, respectively.

RESULTS

(1) The times of writhing body (9.43 +/- 3.87 and 10.28 +/- 4.23) were significantly lower and the latency period of writhing body (12.43 +/- 3.46, 11.00 +/- 3.65) were longer in the group A and the group A+IC as compared with (15.43 +/- 5.13, 17.00 +/- 3.87) and (7.57 +/- 1.99, 8.43 +/- 2.57) in the group M and group A+I (P < 0.05), respectively. (2) The levels of Cx43 mR NA level and protein expression of acupoint in the group A+I were significantly lower than those of the group N (P < 0.05). (3) OTR and VPR mRNA in the uterus in the group A and the group A+IC were significantly lower than those in the group M and the group A+I (P < 0.05), with no significant difference between the group M and the group A+I (P > 0.05). (4) As compared with the group M, PGE2 level increased and PGF2alpha level decreased in the group A and the group A+IC (P < 0.05).

CONCLUSION

Silencing Cx43 expression of partial acupoint can inhibit effectively the effect of acupuncture through decreasing OTR and VPR in endometrium of the dysmenorrheal rat and adjusting the prostaglandins (PGs) synthesis system, which possibly is one of the mechanisms of acupuncture for treatment of primary dysmenorrhea.

摘要

目的

观察部分穴位沉默连接蛋白43（Cx43）表达对针刺效应的影响，以探讨针刺治疗原发性痛经的机制。

方法

采用催产素和RNA干扰（RNAi）技术建立原发性痛经大鼠模型，沉默穴位中Cx43的表达。将50只SD雌性大鼠分为五组，正常组（N）、模型组（M）、针刺组（A）、针刺加干扰组（A+I）、针刺加干扰对照组（A+IC）。采用RT-PCR法观察各组大鼠子宫中催产素受体（OTR）和血管加压素受体（VPR）mRNA的表达。分别采用放射免疫法和ELISA法检测血浆前列腺素E2（PGE2）和前列腺素F2α（PGF2α）水平。

结果

（1）与模型组（15.43±5.13，17.00±3.87）和针刺加干扰组（7.57±1.99，8.43±2.57）相比，针刺组和针刺加干扰对照组的扭体次数（9.43±3.87和10.28±4.23）明显减少，扭体潜伏期（12.43±3.46，11.00±3.65）延长（P<0.05）。（2）针刺加干扰组穴位Cx43 mRNA水平和蛋白表达明显低于正常组（P<0.05）。（3）针刺组和针刺加干扰对照组子宫中OTR和VPR mRNA明显低于模型组和针刺加干扰组（P<0.05），模型组和针刺加干扰组之间无明显差异（P>0.05）。（4）与模型组相比，针刺组和针刺加干扰对照组PGE2水平升高，PGF2α水平降低（P<0.05）。