Moreno-Rojas J M, Tulli F, Messina M, Tibaldi E, Guillou C
Institute for Health and Consumer Protection, Physical and Chemical Exposure Unit, MAST-Group, TP281, Via Fermi 2, 21020 Ispra (VA), Italy.
Rapid Commun Mass Spectrom. 2008 Dec;22(23):3706-10. doi: 10.1002/rcm.3775.
The use of stable isotope ratio analysis (SIRA) as a rapid analytical tool to characterize and discriminate farmed fish on the basis of the feedstuffs included in the diet formulation is discussed. Two isoproteic (44.8%) and isolipidic (19.6%) extruded diets were formulated: a fish-meal-based diet (FM diet), containing fish meal as the sole protein source; a plant-protein-based diet (PP diet), where pea protein concentrate and wheat gluten meal replaced 80% of fish meal protein. The diets were fed to eight groups of rainbow trout (initial body weight: 106.6g) for 103 days in two daily meals under controlled rearing conditions. Growth performance (final body weight: 318.5 g; specific growth rate: 1.06%) and feed-to-gain ratio (0.79) were not affected by the dietary treatment. The differences in isotopic values of the two diets were clearly reflected in the different carbon and nitrogen isotopic values in rainbow trout fillets. The delta(13)C and delta(15)N values of muscle of farmed rainbow trout showed differences between farmed fish fed a fish-protein-based diet (-20.47 +/- 0.34 and 12.38 +/- 0.57 for delta(13)C and delta(15)N, respectively) and those fed a plant-protein-based diet (-23.96 +/- 0.38 and 7.15 +/- 0.51 for delta(13)C and delta(15)N, respectively). The results suggest that SIRA provides a robust and verifiable analytical tool to discriminate between fish fed on a plant or a fish protein diet.
本文讨论了使用稳定同位素比率分析（SIRA）作为一种快速分析工具，根据日粮配方中所含饲料来表征和区分养殖鱼类。配制了两种等蛋白（44.8%）和等脂（19.6%）的挤压日粮：一种以鱼粉为基础的日粮（FM日粮），以鱼粉作为唯一蛋白质来源；一种以植物蛋白为基础的日粮（PP日粮），其中豌豆浓缩蛋白和小麦面筋粉替代了80%的鱼粉蛋白。在可控养殖条件下，将这些日粮分两餐投喂给八组虹鳟鱼（初始体重：106.6克），持续103天。生长性能（最终体重：318.5克；特定生长率：1.06%）和饲料转化率（0.79）不受日粮处理的影响。两种日粮同位素值的差异清楚地反映在虹鳟鱼片不同的碳和氮同位素值上。养殖虹鳟鱼肌肉的δ(13)C和δ(15)N值显示，以鱼蛋白为基础日粮喂养的养殖鱼（δ(13)C和δ(15)N分别为-20.47±0.34和12.38±0.57）与以植物蛋白为基础日粮喂养的养殖鱼（δ(13)C和δ(15)N分别为-23.96±0.38和7.15±0.51）之间存在差异。结果表明，SIRA提供了一种强大且可验证的分析工具，用于区分以植物蛋白或鱼蛋白日粮喂养的鱼类。
