间充质干细胞的化学工程改造以诱导细胞滚动反应。

Chemical engineering of mesenchymal stem cells to induce a cell rolling response.

作者信息

Sarkar Debanjan, Vemula Praveen Kumar, Teo Grace S L, Spelke Dawn, Karnik Rohit, Wee Le Y, Karp Jeffrey M

机构信息

Department of Medicine, Harvard-MIT Division of Heath Sciences and Technology, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, USA.

出版信息

Bioconjug Chem. 2008 Nov 19;19(11):2105-9. doi: 10.1021/bc800345q.

DOI:10.1021/bc800345q

PMID:18973352

Abstract

Covalently conjugated sialyl Lewis X (SLeX) on the mesenchymal stem cell (MSC) surface through a biotin-streptavidin bridge imparts leukocyte-like rolling characteristics without altering the cell phenotype and the multilineage differentiation potential. We demonstrate that the conjugation of SLeX on the MSC surface is stable, versatile, and induces a robust rolling response on P-selectin coated substrates. These results indicate the potential to increase the targeting efficiency of any cell type to specific tissue.

摘要

通过生物素-链霉亲和素桥在间充质干细胞（MSC）表面共价偶联唾液酸化路易斯X（SLeX），可赋予其白细胞样滚动特性，同时不改变细胞表型和多向分化潜能。我们证明，MSC表面SLeX的偶联是稳定、通用的，并能在包被P-选择素的底物上诱导强烈的滚动反应。这些结果表明，提高任何细胞类型对特定组织靶向效率的潜力。