Fletcher J E, Conti P A, Rosenberg H
Department of Anesthesiology, Hahnemann University, Philadelphia.
Acta Anaesthesiol Scand. 1991 Aug;35(6):483-7. doi: 10.1111/j.1399-6576.1991.tb03333.x.
Different in vitro halothane testing procedures have been used in the European malignant hyperthermia (MH) Group Protocol (EMHGP) and the North American MH Group Protocol (NAMHGP), whereas the caffeine-testing protocols are very similar. The present study compares the two halothane-testing protocols in ten MH susceptible swine and in four control swine. Halothane contracture testing was conducted in vitro 12-52 days following the barnyard challenge that established the MH susceptibility of the swine. There was one false positive and one false negative halothane test by the EMHGP. The MH-equivocal category in the EMHGP, which is treated clinically as MH-susceptible, affords a margin of safety in such cases. In contrast, there were no false halothane tests by the NAMHGP. While some skeletal muscle strips from MH pigs were normal by both protocols (NAMHGP 30%; EMHGP 10%), the outcome of halothane testing by the NAMHGP was unaffected. The response to halothane 3% is reduced if preceded by the EMHGP, suggesting that simply adding halothane 3% to the end of the EMHGP does not permit a direct quantitative comparison to the NAMHGP. However, the diagnostic outcomes of the two approaches are similar.
欧洲恶性高热（MH）研究组方案（EMHGP）和北美MH研究组方案（NAMHGP）采用了不同的体外氟烷测试程序，而咖啡因测试方案非常相似。本研究比较了10头MH易感性猪和4头对照猪的两种氟烷测试方案。在确定猪的MH易感性的猪场激发试验后12 - 52天进行体外氟烷挛缩试验。EMHGP的氟烷试验有1例假阳性和1例假阴性。EMHGP中临床上被视为MH易感性的MH可疑类别在这种情况下提供了安全边际。相比之下，NAMHGP没有氟烷试验假阳性。虽然两种方案中来自MH猪的一些骨骼肌条均正常（NAMHGP为30%；EMHGP为10%），但NAMHGP的氟烷测试结果不受影响。如果在EMHGP之前进行，则对3%氟烷的反应会降低，这表明仅在EMHGP末尾添加3%氟烷不允许与NAMHGP进行直接定量比较。然而，两种方法的诊断结果相似。
