Na Yi-Rang, Seok Seung-Hyeok, Baek Min-Won, Lee Hui-Young, Kim Dong-Jae, Park Sung-Hoon, Lee Hyun-Kyoung, Park Jae-Hak
Department of Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University, Seoul 151-742, Republic of Korea.
Ecotoxicol Environ Saf. 2009 Mar;72(3):714-9. doi: 10.1016/j.ecoenv.2008.09.015. Epub 2008 Oct 29.
3,3',4,4',5-Pentachlorinated biphenyls 126 (PCB126) is a global environmental contaminant that can induce cellular oxidative stress. We investigated whether vitamin E can protect against toxicity from PCB126 during zebrafish (Danio rerio) development. Zebrafish embryos were exposed to 100nM PCB126 and compared with a second group that was co-exposed with 100muM vitamin E until 5 days post fertilization. PCB126 induced pericardial sac edema, yolk sac edema, and growth retardation in zebrafish embyos. In contrast, vitamin E co-exposure group did not show any gross changes. Real-time PCR results showed that vitamin E co-exposure group were restored to control group for the expression levels of heat shock protein 70 Cognate, aryl hydrocarbon receptor type-2, cytochrome P450 1A, and superoxide dismutase-1. These data give insights into the use of vitamin E to reduce PCB126-mediated toxicity and into the use of zebrafish embryos for exploring mechanisms underlying the oxidative potential of AHR agonists.
3,3',4,4',5-五氯联苯126（PCB126）是一种可诱发细胞氧化应激的全球环境污染物。我们研究了维生素E是否能在斑马鱼（Danio rerio）发育过程中抵御PCB126的毒性。将斑马鱼胚胎暴露于100nM的PCB126中，并与另一组在受精后5天内同时暴露于100μM维生素E的胚胎进行比较。PCB126可诱发斑马鱼胚胎的心包囊水肿、卵黄囊水肿和生长迟缓。相比之下，同时暴露于维生素E的组未出现任何明显变化。实时聚合酶链反应结果显示，同时暴露于维生素E的组中，热休克蛋白70同源物、芳烃受体2型、细胞色素P450 1A和超氧化物歧化酶-1的表达水平恢复到了对照组水平。这些数据为利用维生素E降低PCB126介导的毒性以及利用斑马鱼胚胎探索AHR激动剂氧化潜力的潜在机制提供了见解。
