小鼠皮肤伤口愈合过程中基因表达的时间进程分析。
The time-course analysis of gene expression during wound healing in mouse skin.
作者信息
Kagawa Shinichiro, Matsuo Aya, Yagi Yoichi, Ikematsu Kazuya, Tsuda Ryouichi, Nakasono Ichiro
机构信息
Department of Forensic Pathology and Science, Unit of Social Medicine, Course of Medical and Dental Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Sakamoto, Nagazaki, Japan.
出版信息
Leg Med (Tokyo). 2009 Mar;11(2):70-5. doi: 10.1016/j.legalmed.2008.09.004. Epub 2008 Oct 29.
RNA analysis has been applied to forensic work to determine wound age. We investigated mRNA expression using quantitative RT-PCR of ten genes, including c-fos, fosB, mitogen-activated protein kinase phosphatase-1 (MKP-1), CD14, chemokine (C-C motif) ligand 9 (CCL9), placenta growth factor (PlGF), mast cell protease-5 (MCP-5), growth arrest specific 5 (Gas5), beta-2 microglobulin (B2M) and major urinary protein-1 (MUP-1), in terms of repair response in adult mice. The expression level of c-fos, fosB and MKP-1 transcripts increased drastically, peaked within 1h, and that of the CD14 and CCL9 transcripts peaked from 12 to 24h. An increase in PlGF and MCP-5 mRNA appeared on about day 5. Gas5, B2M and MUP-1 transcripts showed no significant change. Each gene had differentially expressional patterns with time-course. Our result implied that the observation of the 7 genes in wounded skin could serve to aid in the accurate diagnosis of wound age.
RNA分析已应用于法医工作以确定伤口年龄。我们使用定量逆转录聚合酶链反应（RT-PCR）研究了成年小鼠十个基因的mRNA表达，这些基因包括c-fos、fosB、丝裂原活化蛋白激酶磷酸酶-1（MKP-1）、CD14、趋化因子（C-C基序）配体9（CCL9）、胎盘生长因子（PlGF）、肥大细胞蛋白酶-5（MCP-5）、生长停滞特异性蛋白5（Gas5）、β2微球蛋白（B2M）和主要尿蛋白-1（MUP-1），以了解其在修复反应方面的情况。c-fos、fosB和MKP-1转录本的表达水平急剧上升，在1小时内达到峰值，而CD14和CCL9转录本的峰值出现在12至24小时。PlGF和MCP-5 mRNA在大约第5天出现增加。Gas5、B2M和MUP-1转录本未显示出显著变化。每个基因都有随时间变化的差异表达模式。我们的结果表明，观察伤口皮肤中的这7个基因有助于准确诊断伤口年龄。
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