Ackroyd E B, Man W K, Simpkin S, Parkin J V, Green C J
Section of Surgical Research, MRC Clinical Research Centre, Harrow, Middlesex, UK.
Agents Actions. 1991 May;33(1-2):157-60. doi: 10.1007/BF01993154.
During the course of a study on preservation of small bowel transplants in rats, the hypothesis that histamine may play a role in graft damage has been investigated. Plasma and mucosal histamine levels have been measured after storage and reperfusion of Lewis rat small bowel transplants which have received an intravascular flush of saline or of one of the tissue preservation media, hypertonic citrate or University of Wisconsin solution. Plasma histamine concentration was unchanged from a control value of 23.2 +/- 2.6 ng/ml 15 min after reperfusion of grafts, whether fresh or stored for 24 h or for 48 h. Mucosal histamine levels in the grafts fell, however, from a control value of 371.0 +/- 22.9 ng/g tissue, first on storage then further after 15 min reperfusion. No differences were found in these parameters of histamine release between any of the preservation media. It is suggested that histamine may play a role in storage and reperfusion damage to small bowel transplants.
在一项关于大鼠小肠移植保存的研究过程中，对组胺可能在移植物损伤中起作用这一假说进行了研究。在对接受盐水或其中一种组织保存液（高渗枸橼酸盐或威斯康星大学溶液）血管内冲洗的Lewis大鼠小肠移植进行储存和再灌注后，测定了血浆和黏膜组胺水平。无论移植物是新鲜的、储存24小时还是48小时，再灌注15分钟后血浆组胺浓度与23.2±2.6 ng/ml的对照值相比均无变化。然而，移植物中的黏膜组胺水平从371.0±22.9 ng/g组织的对照值下降，先是在储存时下降，然后在再灌注15分钟后进一步下降。在任何一种保存液之间，这些组胺释放参数均未发现差异。提示组胺可能在小肠移植的储存和再灌注损伤中起作用。
