Franco Fraguas L, Carlsson J, Lönnberg M
Cátedra de Bioquímica, Departamento de Biociencias, Facultad de Quimica, CC 1157, Montevideo, Uruguay.
J Chromatogr A. 2008 Nov 28;1212(1-2):82-8. doi: 10.1016/j.chroma.2008.10.036. Epub 2008 Oct 17.
This work exploits the combination of the lectin affinity chromatography (LAC) with an ultra-sensitive immunochromatographic assay to differentiate several types of erythropoietin (EPO). The chromatographic behaviours of different commercial types of recombinant human EPO (rhEPO), EPO analogues (Aranesp) and urine human EPO (uhEPO) from healthy individuals on eight lectin-Sepharose columns, have been worked out. Results show that when using wheat germ agglutinin (WGA)-Sepharose columns, a careful desorption regime starting with very low concentration (2mM) of the competitive sugar N-acetylglucosamine (GlcNAc) makes it possible to efficiently distinguish endogenous EPO from recombinant EPO and EPO analogues.
这项工作利用凝集素亲和色谱法（LAC）与超灵敏免疫色谱分析相结合的方法来区分几种类型的促红细胞生成素（EPO）。研究了不同商业类型的重组人促红细胞生成素（rhEPO）、促红细胞生成素类似物（阿法依泊汀）以及健康个体的尿促红细胞生成素（uhEPO）在八种凝集素-琼脂糖柱上的色谱行为。结果表明，当使用麦胚凝集素（WGA）-琼脂糖柱时，从极低浓度（2mM）的竞争性糖类N-乙酰葡糖胺（GlcNAc）开始的仔细解吸方案能够有效地将内源性EPO与重组EPO和EPO类似物区分开来。
